Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis

doi:10.1186/s12870-018-1566-0

	Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis
	Liu, Jie 2; Moyankova, Daniela 1; Lin, Chih-Ta ; Mladenov, Petko 1; Sun, Run-Ze ; Djilianov, Dimitar 1; Deng, Xin
	2018
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	18
摘要	BackgroundWater shortage is a major factor that harms agriculture and ecosystems worldwide. Plants display various levels of tolerance to water deficit, but only resurrection plants can survive full desiccation of their vegetative tissues. Haberlea rhodopensis, an endemic plant of the Balkans, is one of the few resurrection plants found in Europe. We performed transcriptomic analyses of this species under slight, severe and full dehydration and recovery to investigate the dynamics of gene expression and associate them with existing physiological and metabolomics data.ResultsDe novo assembly yielded a total of 142,479 unigenes with an average sequence length of 1034nt. Among them, 18,110 unigenes were differentially expressed. Hierarchical clustering of all differentially expressed genes resulted in seven clusters of dynamic expression patterns. The most significant expression changes, involving more than 15,000 genes, started at severe dehydration (similar to 20% relative water content) and were partially maintained at full desiccation (<10% relative water content). More than a hundred pathways were enriched and functionally organized in a GO/pathway network at the severe dehydration stage. Transcriptomic changes in key pathways were analyzed and discussed in relation to metabolic processes, signal transduction, quality control of protein and DNA repair in this plant during dehydration and rehydration.ConclusionReprograming of the transcriptome occurs during severe dehydration, resulting in a profound alteration of metabolism toward alternative energy supply, hormone signal transduction, and prevention of DNA/protein damage under very low cellular water content, underlying the observed physiological and metabolic responses and the resurrection behavior of H. rhodopensis.
关键词	Desiccation tolerance Haberlea rhodopensis Hormone signaling pathway Primary and secondary metabolism Resurrection plant Transcriptome
学科领域	Plant Sciences
DOI	10.1186/s12870-018-1566-0
收录类别	SCI
语种	英语
WOS关键词	ACID BETA-OXIDATION ; DESICCATION-TOLERANCE ; QUALITY-CONTROL ; CELL-DEATH ; PROTEIN ; GENE ; DNA ; ANNOTATION ; DROUGHT ; GENOME
WOS记录号	WOS:000452979500001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	China-Bulgaria's Inter-Governmental S&T Cooperation project [14-10] ; Bulgarian Science FundNational Science Fund of Bulgaria [DNTS/China/01/7]
作者邮箱	d_djilianov@abi.bg ; deng@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20384
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Agricultural Acad, Agrobioinst, Abiot Stress Grp, Sofia 1164, Bulgaria 3.Weifang Univ Sci & Technol, Facil Hort Lab Univ Shandong, Shouguang 262700, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jie,Moyankova, Daniela,Lin, Chih-Ta,et al. Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis[J]. BMC PLANT BIOLOGY,2018,18.
APA	Liu, Jie.,Moyankova, Daniela.,Lin, Chih-Ta.,Mladenov, Petko.,Sun, Run-Ze.,...&Deng, Xin.(2018).Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis.BMC PLANT BIOLOGY,18.
MLA	Liu, Jie,et al."Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis".BMC PLANT BIOLOGY 18(2018).

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