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| Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis | |
Liu, Jie2; Moyankova, Daniela1; Lin, Chih-Ta; Mladenov, Petko1; Sun, Run-Ze; Djilianov, Dimitar1; Deng, Xin
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| 2018 | |
| Source Publication | BMC PLANT BIOLOGY
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| ISSN | 1471-2229 |
| Volume | 18 |
| Abstract | BackgroundWater shortage is a major factor that harms agriculture and ecosystems worldwide. Plants display various levels of tolerance to water deficit, but only resurrection plants can survive full desiccation of their vegetative tissues. Haberlea rhodopensis, an endemic plant of the Balkans, is one of the few resurrection plants found in Europe. We performed transcriptomic analyses of this species under slight, severe and full dehydration and recovery to investigate the dynamics of gene expression and associate them with existing physiological and metabolomics data.ResultsDe novo assembly yielded a total of 142,479 unigenes with an average sequence length of 1034nt. Among them, 18,110 unigenes were differentially expressed. Hierarchical clustering of all differentially expressed genes resulted in seven clusters of dynamic expression patterns. The most significant expression changes, involving more than 15,000 genes, started at severe dehydration (similar to 20% relative water content) and were partially maintained at full desiccation (<10% relative water content). More than a hundred pathways were enriched and functionally organized in a GO/pathway network at the severe dehydration stage. Transcriptomic changes in key pathways were analyzed and discussed in relation to metabolic processes, signal transduction, quality control of protein and DNA repair in this plant during dehydration and rehydration.ConclusionReprograming of the transcriptome occurs during severe dehydration, resulting in a profound alteration of metabolism toward alternative energy supply, hormone signal transduction, and prevention of DNA/protein damage under very low cellular water content, underlying the observed physiological and metabolic responses and the resurrection behavior of H. rhodopensis. |
| Keyword | Desiccation tolerance Haberlea rhodopensis Hormone signaling pathway Primary and secondary metabolism Resurrection plant Transcriptome |
| Subject Area | Plant Sciences |
| DOI | 10.1186/s12870-018-1566-0 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ACID BETA-OXIDATION ; DESICCATION-TOLERANCE ; QUALITY-CONTROL ; CELL-DEATH ; PROTEIN ; GENE ; DNA ; ANNOTATION ; DROUGHT ; GENOME |
| WOS ID | WOS:000452979500001 |
| Publisher | BMC |
| Subtype | Article |
| Publication Place | LONDON |
| Funding Organization | China-Bulgaria's Inter-Governmental S&T Cooperation project [14-10] ; Bulgarian Science FundNational Science Fund of Bulgaria [DNTS/China/01/7] |
| Corresponding Author Email | d_djilianov@abi.bg ; deng@ibcas.ac.cn |
| OA | Green Published, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/20384 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Agricultural Acad, Agrobioinst, Abiot Stress Grp, Sofia 1164, Bulgaria 3.Weifang Univ Sci & Technol, Facil Hort Lab Univ Shandong, Shouguang 262700, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Jie,Moyankova, Daniela,Lin, Chih-Ta,et al. Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis[J]. BMC PLANT BIOLOGY,2018,18. |
| APA | Liu, Jie.,Moyankova, Daniela.,Lin, Chih-Ta.,Mladenov, Petko.,Sun, Run-Ze.,...&Deng, Xin.(2018).Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis.BMC PLANT BIOLOGY,18. |
| MLA | Liu, Jie,et al."Transcriptome reprogramming during severe dehydration contributes to physiological and metabolic changes in the resurrection plant Haberlea rhodopensis".BMC PLANT BIOLOGY 18(2018). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Liu-2018-Transcripto（2259KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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