Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis

doi:10.1186/s12870-018-1363-9

	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis
	Yang, Zhenle ; Liu, Dantong 1; Ji, Hongying 1
	2018
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	18
摘要	Background: Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots. Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues. In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil-or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism. Results: The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development. The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B-and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway. Conclusions: We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue. The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.
关键词	Cyperus esculentus Oil-rich tuber Sucrose metabolism Enzymatic pathways Gene expression pattern
学科领域	Plant Sciences
DOI	10.1186/s12870-018-1363-9
收录类别	SCI
语种	英语
WOS关键词	SYNTHASE GENE FAMILY ; PHOSPHATE SYNTHASE ; ARABIDOPSIS-THALIANA ; SEED DEVELOPMENT ; ACID INVERTASE ; CARBOHYDRATE-METABOLISM ; DEVELOPMENTAL-CHANGES ; ENDOSPERM DEVELOPMENT ; EXPRESSION ANALYSIS ; SUGAR ACCUMULATION
WOS记录号	WOS:000439727600002
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	Beijing Natural Science FoundationBeijing Natural Science Foundation [5151001] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371692]
作者邮箱	yangzhl@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20385
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Zhenle,Liu, Dantong,Ji, Hongying. Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis[J]. BMC PLANT BIOLOGY,2018,18.
APA	Yang, Zhenle,Liu, Dantong,&Ji, Hongying.(2018).Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.BMC PLANT BIOLOGY,18.
MLA	Yang, Zhenle,et al."Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis".BMC PLANT BIOLOGY 18(2018).

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