MADS-box family genes in sheepgrass and their involvement in abiotic stress responses

doi:10.1186/s12870-018-1259-8

	MADS-box family genes in sheepgrass and their involvement in abiotic stress responses
	Jia, Junting 1; Zhao, Pincang 2; Cheng, Liqin ; Yuan, Guangxiao ; Yang, Weiguang 1,3; Liu, Shu 1; Chen, Shuangyan ; Qi, Dongmei ; Liu, Gongshe; Li, Xiaoxia
	2018
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	18
摘要	Background: MADS-box genes are categorized into A, B, C, D and E classes and are involved in floral organ identity and flowering. Sheepgrass (Leymus chinensis (Trin.) Tzvel) is an important perennial forage grass and adapts well to many adverse environments. However, there are few studies on the molecular mechanisms of flower development in sheepgrass, especially studies on MADS-domain proteins. Results: In this study, we cloned 11 MADS-box genes from sheepgrass (Leymus chinensis (Trin.) Tzvel), and phylogenetic analysis of the 11 genes with their homologs revealed that they are divided into nine subclades. Tissue-specific expression profile analysis showed that most of these MADS-box genes were highly expressed in floral organs. LcMADS1 and LcMADS3 showed higher expression in the stamen than in the other tissues, and LcMADS7 showed high expression in the stamen, glume, lemma and palea, while expression of LcMADS2, LcMADS9 and LcMADS11 was higher in vegetative organs than floral organs. Furthermore, yeast two-hybrid analyses showed that LcMADS2 interacted with LcMADS7 and LcMADS9. LcMADS3 interacted with LcMADS4, LcMADS7 and LcMADS10, while LcMADS1 could interact with only LcMADS7. Interestingly, the expression of LcMADS1 and LcMADS2 were significantly induced by cold, and LcMADS9 was significantly up-regulated by NaCl. Conclusion: Hence, we proposed that LcMADS1, LcMADS2, LcMADS3, LcMADS7 and LcMADS9 play a pivotal role in sheepgrass sexual reproduction and may be involved in abiotic stress responses, and our findings provide useful information for further exploration of the functions of this gene family in rice, wheat and other graminaceous cereals.
关键词	MADS-box genes Sheepgrass Abiotic stress Gene expression Sexual reproduction Yeast two-hybrid assay
学科领域	Plant Sciences
DOI	10.1186/s12870-018-1259-8
收录类别	SCI
语种	英语
WOS关键词	FLOWER DEVELOPMENT ; FLORAL ORGAN ; FUNCTIONAL CONSERVATION ; TRANSCRIPTION FACTOR ; PROTEIN INTERACTIONS ; DOMAIN PROTEINS ; RICE ; EXPRESSION ; MERISTEM ; INFLORESCENCE
WOS记录号	WOS:000427947800001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Basic Research Program of China ('973')National Basic Research Program of China [2014CB138704] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences (STS) [KFJ-EW-STS-119]
作者邮箱	liugs@ibcas.ac.cn ; lixx2013@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20387
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Huaihua Univ, Coll Biol & Food Engn, Huaihua 418000, Hunan, Peoples R China 4.Inst Anim Sci Heilongjiang Prov, Qiqihar, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Jia, Junting,Zhao, Pincang,Cheng, Liqin,et al. MADS-box family genes in sheepgrass and their involvement in abiotic stress responses[J]. BMC PLANT BIOLOGY,2018,18.
APA	Jia, Junting.,Zhao, Pincang.,Cheng, Liqin.,Yuan, Guangxiao.,Yang, Weiguang.,...&Li, Xiaoxia.(2018).MADS-box family genes in sheepgrass and their involvement in abiotic stress responses.BMC PLANT BIOLOGY,18.
MLA	Jia, Junting,et al."MADS-box family genes in sheepgrass and their involvement in abiotic stress responses".BMC PLANT BIOLOGY 18(2018).

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