Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction

doi:10.1038/s41598-018-21195-2

IB-CAS > 中科院光生物学重点实验室

	Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction
	Magyar, Melinda ; Sipka, Gabor ; Kovacs, Laszlo ; Ughy, Bettina ; Zhu, Qingjun ; Han, Guangye ; Spunda, Vladimir 2,3; Lambrev, Petar H.; Shen, Jian-Ren; Garab, Gyozo 2
	2018
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8
摘要	Photosystem II (PSII) catalyses the photoinduced oxygen evolution and, by producing reducing equivalents drives, in concert with PSI, the conversion of carbon dioxide to sugars. Our knowledge about the architecture of the reaction centre (RC) complex and the mechanisms of charge separation and stabilisation is well advanced. However, our understanding of the processes associated with the functioning of RC is incomplete: the photochemical activity of PSII is routinely monitored by chlorophyll-a fluorescence induction but the presently available data are not free of controversy. In this work, we examined the nature of gradual fluorescence rise of PSII elicited by trains of singleturnover saturating flashes (STSFs) in the presence of a PSII inhibitor, permitting only one stable charge separation. We show that a substantial part of the fluorescence rise originates from lightinduced processes that occur after the stabilisation of charge separation, induced by the first STSF; the temperature-dependent relaxation characteristics suggest the involvement of conformational changes in the additional rise. In experiments using double flashes with variable waiting times (Delta tau) between them, we found that no rise could be induced with zero or short Delta tau, the value of which depended on the temperature -revealing a previously unknown rate-limiting step in PSII.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41598-018-21195-2
收录类别	SCI
语种	英语
WOS关键词	OXYGENIC PHOTOSYNTHESIS ; CONFORMATIONAL-CHANGE ; PROTEIN FLEXIBILITY ; HARVESTING COMPLEX ; ENERGY-CONVERSION ; ELECTRON-TRANSFER ; REACTION CENTERS ; CHLOROPLASTS ; YIELD ; TEMPERATURE
WOS记录号	WOS:000424634000009
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	Strategic Priority Research ProgramChinese Academy of Sciences [XDB17030100] ; Key Research Project for Frontier Science from CAS, China [QYZDY-SSW-SMC003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 112688, KH 124985, TET_15-1-2016-0144, GINOP-2.3.2-15-2016-00058, OTKA PD 121225] ; Moravian-Silesian Region (Project title: Strengthening international cooperation in science, research and education) [01211/2016/RRC]
作者邮箱	garab.gyozo@brc.mta.hu
作品OA属性	Green Accepted, Green Published, gold
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20391
专题	中科院光生物学重点实验室
作者单位	1.Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, Temesvari Korut 62, H-6726 Szeged, Hungary 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 3.Univ Ostrava, Fac Sci, Dept Phys, Chittussiho 10, CZ-71000 Ostrava, Czech Republic 4.Czech Acad Sci, Global Change Res Inst, Belidla 986-4a, Brno 60300, Czech Republic 5.Okayama Univ, Res Inst Interdisciplinary Sci, 1-1,Naka 3 Chome, Tsushima, Okayama 7008530, Japan 6.Okayama Univ, Grad Sch Nat Sci & Technol, 1-1,Naka 3 Chome, Tsushima, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Magyar, Melinda,Sipka, Gabor,Kovacs, Laszlo,et al. Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction[J]. SCIENTIFIC REPORTS,2018,8.
APA	Magyar, Melinda.,Sipka, Gabor.,Kovacs, Laszlo.,Ughy, Bettina.,Zhu, Qingjun.,...&Garab, Gyozo.(2018).Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction.SCIENTIFIC REPORTS,8.
MLA	Magyar, Melinda,et al."Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction".SCIENTIFIC REPORTS 8(2018).

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