Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator

doi:10.1128/JVI.01004-18

	Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
	Jiang, Jian ; Smith, Heather N.; Ren, Di ; Mudiyanselage, Shachinthaka D. Dissanayaka ; Dawe, Angus L.; Wang, Lei1,2 ; Wang, Ying
	2018
发表期刊	JOURNAL OF VIROLOGY
ISSN	0022-538X
卷号	92 期号:20
摘要	Viroids are circular noncoding RNAs (ncRNAs) that infect plants. Despite differences in the genetic makeup and biogenesis, viroids and various long ncRNAs all rely on RNA structure-based interactions with cellular factors for function. Viroids replicating in the nucleus utilize DNA-dependent RNA polymerase II for transcription, a process that involves a unique splicing form of transcription factor IIIA (TFIIIA-7ZF). Here, we provide evidence showing that potato spindle tuber viroid (PSTVd) interacts with a TFIIIA splicing regulator (ribosomal protein L5 [RPL5]) in vitro and in vivo. PSTVd infection compromises the regulatory role of RPL5 over splicing of TFIIIA transcripts, while ectopic expression of RPL5 reduces TFIIIA-7ZF expression and attenuates PSTVd accumulation. Furthermore, we illustrate that the RPL5 binding site on the PSTVd genome resides in the central conserved region critical for replication. Together, our data suggest that viroids can regulate their own replication and modulate specific regulatory factors leading to splicing changes in only one or a few genes. This study also has implications for understanding the functional mechanisms of ncRNAs and elucidating the global splicing changes in various host-pathogen interactions. IMPORTANCE Viroids are the smallest replicons among all living entities. As circular noncoding RNAs, viroids can replicate and spread in plants, often resulting in disease symptoms. Potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids, requires a unique splicing form of transcription factor IIIA (TFIIIA7ZF) for its propagation. Here, we provide evidence showing that PSTVd directly interacts with a splicing regulator, RPL5, to favor the expression of TFIIIA-7ZF, thereby promoting viroid replication. This finding provides new insights to better understand viroid biology and sheds light on the noncoding RNA-based regulation of splicing. Our discovery also establishes RPL5 as a novel negative factor regulating viroid replication in the nucleus and highlights a potential means for viroid control.
关键词	Viroid noncoding RNA alternative splicing host-viroid interactions
学科领域	Virology
DOI	10.1128/JVI.01004-18
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR IIIA ; RNA-POLYMERASE-II ; NICOTIANA-BENTHAMIANA ; SEQUENCE ; EXPRESSION ; RESISTANCE ; REVEALS ; BIOLOGY ; TOBACCO ; PSTVD
WOS记录号	WOS:000445668400018
出版者	AMER SOC MICROBIOLOGY
文献子类	Article
出版地	WASHINGTON
EISSN	1098-5514
资助机构	U.S. National Science FoundationNational Science Foundation (NSF) [IOS-1564366] ; Strategic Research Initiative at the College of Arts and Sciences, Mississippi State University
作者邮箱	wang@biology.msstate.edu
作品OA属性	Green Published, Bronze
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20392
专题	中科院植物分子生理学重点实验室
作者单位	1.Mississippi State Univ, Dept Biol Sci, Mississippi State, MS 39762 USA 2.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Jian,Smith, Heather N.,Ren, Di,et al. Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator[J]. JOURNAL OF VIROLOGY,2018,92(20).
APA	Jiang, Jian.,Smith, Heather N..,Ren, Di.,Mudiyanselage, Shachinthaka D. Dissanayaka.,Dawe, Angus L..,...&Wang, Ying.(2018).Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator.JOURNAL OF VIROLOGY,92(20).
MLA	Jiang, Jian,et al."Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator".JOURNAL OF VIROLOGY 92.20(2018).

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