IB-CAS  > 系统与进化植物学国家重点实验室
Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae
Frericks, Jonathan; Munkacsi, Andrew1; Ritchie, Peter1; Luo, Yi-Bo; Lehnebach, Carlos A.
2018
发表期刊NEW ZEALAND JOURNAL OF BOTANY
ISSN0028-825X
卷号56期号:1页码:91-108
摘要The New Zealand ladies' tresses Spiranthes novae-zelandiae is a terrestrial orchid currently classified as Threatened - Nationally Vulnerable. Although considered endemic to New Zealand, morphological similarity with the widespread Eurasian Spiranthes sinensis has cast doubt on its taxonomic and biogeographic status. Habitat destruction is the main threat to the survival of S.novae-zelandiae. Unfortunately, lack of information regarding its symbiotic fungal associates and technical expertise within New Zealand with symbiotic seed germination techniques have hindered its propagation from seed. In this study we examined the phylogenetic affinities of S.novae-zelandiae using nuclear (internal transcribed spacer) and chloroplast (trnL, trnS-G and matK) DNA sequences. We also explored the diversity of fungal symbionts associated with S.novae-zelandiae and identified the fungal symbiont that promotes seed germination and seedling development using DNA sequences and in vitro seed germination experiments. Bayesian Inference analyses showed that S.novae-zelandiae is nested within S.sinensis along with Spiranthes australis and Spiranthes aff. novae-zelandiae (CHR 518297; Motutangi), a morphological variant of uncertain taxonomic status from northern New Zealand. These results support earlier suggestions that a broader concept of S.sinensis is needed to include S.novae-zelandiae and many other taxa segregated from S.sinensis. Nine fungal Operational Taxonomic Units were isolated from the roots of S.novae-zelandiae but only one promoted seed germination and seedling development. DNA sequence analyses confirmed that this isolate was a strain of Tulasnella (anamorph: Epulorhiza); a widespread mycorrhizal fungus previously found in S.australis and S.sinensis. Lastly, we describe the germination process and the steps we followed to obtain flowering plants after 2 years of seed inoculation.
关键词Conservation germination mycorrhiza New Zealand orchid phylogeny taxonomy Tulasnella Spiranthes
学科领域Plant Sciences
DOI10.1080/0028825X.2017.1418398
收录类别SCI
语种英语
WOS关键词MYCORRHIZAL SPECIFICITY ; TERRESTRIAL ORCHIDS ; MOLECULAR VARIATION ; FUNGI ; GENUS ; RHIZOCTONIA ; ENDOPHYTES ; SEQUENCES ; ROOTS
WOS记录号WOS:000425492000007
出版者TAYLOR & FRANCIS LTD
文献子类Article
出版地ABINGDON
EISSN1175-8643
资助机构Wellington Botanical Society ; San Diego County Orchid Society (USA) ; Australian Orchid Foundation [287/2012] ; Te Papa MSc Scholarship in Plant Molecular Systematics
作者邮箱carlosl@tepapa.govt.nz
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/20419
专题系统与进化植物学国家重点实验室
作者单位1.[Frericks, Jonathan; Lehnebach, Carlos A.] Museum New Zealand Te Papa Tongarewa, Wellington, New Zealand
2.Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand
3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Frericks, Jonathan,Munkacsi, Andrew,Ritchie, Peter,et al. Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae[J]. NEW ZEALAND JOURNAL OF BOTANY,2018,56(1):91-108.
APA Frericks, Jonathan,Munkacsi, Andrew,Ritchie, Peter,Luo, Yi-Bo,&Lehnebach, Carlos A..(2018).Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae.NEW ZEALAND JOURNAL OF BOTANY,56(1),91-108.
MLA Frericks, Jonathan,et al."Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae".NEW ZEALAND JOURNAL OF BOTANY 56.1(2018):91-108.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Phylogenetic affinit(2984KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Frericks, Jonathan]的文章
[Munkacsi, Andrew]的文章
[Ritchie, Peter]的文章
百度学术
百度学术中相似的文章
[Frericks, Jonathan]的文章
[Munkacsi, Andrew]的文章
[Ritchie, Peter]的文章
必应学术
必应学术中相似的文章
[Frericks, Jonathan]的文章
[Munkacsi, Andrew]的文章
[Ritchie, Peter]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Phylogenetic affinities and in vitro seed germ.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。