The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

doi:10.1104/pp.17.01449

	The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes
	Li, Shan 1; Xu, Huijinlan ; Ju, Zheng ; Cao, Dongyan ; Zhu, Hongliang ; Fu, Daqi ; Grierson, Donald 2,3; Qin, Guozheng4 ; Luo, Yunbo ; Zhu, Benzhong
	2018
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	176 期号:1 页码:891-909
摘要	Fruit development and ripening is regulated by genetic and environmental factors and is of critical importance for seed dispersal, reproduction, and fruit quality. Tomato (Solanum lycopersicum) ripening inhibitor (rin) mutant fruit have a classic ripening-inhibited phenotype, which is attributed to a genomic DNA deletion resulting in the fusion of two truncated transcription factors, RIN and MC. In wild-type fruit, RIN, aMADS-box transcription factor, is a key regulator of the ripening gene expression network, with hundreds of gene targets controlling changes in color, flavor, texture, and taste during tomato fruit ripening; MC, on the other hand, has low expression in fruit, and the potential functions of the RIN-MC fusion gene in ripening remain unclear. Here, overexpression of RIN-MC in transgenic wild-type cv Ailsa Craig tomato fruits impaired several ripening processes, and down-regulating RIN-MC expression in the rin mutant was found to stimulate the normal yellow mutant fruit to produce a weak red color, suggesting a distinct negative role for RIN-MC in tomato fruit ripening. By comparative transcriptome analysis of rin and rin 35S:: RIN-MC RNA interference fruits, a total of 1,168 and 1,234 genes were identified as potential targets of RIN-MC activation and inhibition. Furthermore, the RIN-MC fusion gene was shown to be translated into a chimeric transcription factor that was localized to the nucleus and was capable of protein interactions with other MADS-box factors. These results indicated that tomato RIN-MC fusion plays a negative role in ripening and encodes a chimeric transcription factor that modulates the expression of many ripening genes, thereby contributing to the rin mutant phenotype.
学科领域	Plant Sciences
DOI	10.1104/pp.17.01449
收录类别	SCI
语种	英语
WOS关键词	TOMATO FRUITFULL HOMOLOGS ; TRANSIENT EXPRESSION ; PROTEIN INTERACTIONS ; ETHYLENE ; DOMAIN ; IDENTIFICATION ; REGULATOR ; ARABIDOPSIS ; EVOLUTION ; GENETICS
WOS记录号	WOS:000419675300068
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31571894, 31271959] ; Education Foundation of Da Bei Nong Group [1061-2415002]
作者邮箱	lyb@cau.edu.cn ; zbz@cau.edu.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：90[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20425
专题	中科院北方资源植物重点实验室
作者单位	1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China 2.Hubei Engn Univ, Hubei Prov Res Ctr Engn Technol Utilizat Bot Func, Xiaogan 432000, Peoples R China 3.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China 4.Univ Nottingham, Sch Biosci, Div Plant & Crop Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Shan,Xu, Huijinlan,Ju, Zheng,et al. The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes[J]. PLANT PHYSIOLOGY,2018,176(1):891-909.
APA	Li, Shan.,Xu, Huijinlan.,Ju, Zheng.,Cao, Dongyan.,Zhu, Hongliang.,...&Zhu, Benzhong.(2018).The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes.PLANT PHYSIOLOGY,176(1),891-909.
MLA	Li, Shan,et al."The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes".PLANT PHYSIOLOGY 176.1(2018):891-909.

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