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Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains
Xue, Yiqun1; Xing, Jingjing1; Wan, Yinglang; Lv, Xueqin; Fan, Lusheng2; Zhang, Yongdeng3; Song, Kai1; Wang, Li; Wang, Xiaohua; Deng, Xin; Baluska, Frantisek4; Christie, John M.; Lin, Jinxing6
Source PublicationMOLECULAR PLANT
AbstractPhototropin (phot)-mediated signaling initiated by blue light (BL) plays a critical role in optimizing photosynthetic light capture at the plasma membrane (PM) in plants. However, the mechanisms underlying the regulation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and stepwise photobleaching analysis of phot1-GFP proteins we demonstrated that in the dark phot1 proteins remain in an inactive state and mostly exist as monomers. Dimerization and the diffusion rate of phot1-GFP increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive phot1(D806N) -GFP but did enhance its dimerization, suggesting that phot1 dimerization is independent of phosphorylation. Forster resonance energy transfer-fluorescence lifetime imaging microscopy analysis revealed that the interaction between phot1-GFP and a marker of sterolrich lipid environments, AtRem1.3-mCherry, was enhanced with increased time of BL treatment. However, this BL-dependent interaction was not obvious in plants co-expressing phot1(D806N)-GFP and AtRem1.3-mCherry, indicating that BL facilitates the translocation of functional phot1-GFP into AtRem1.3-labeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated phot1-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane micro-domains act as organizing platforms essential for the proper function of activated phot1 at the PM.
Keywordphot1 VA-TIRFM spatiotemporal dynamics blue light signaling membrane microdomains
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Indexed BySCI
WOS IDWOS:000434429000008
Publication PlaceCAMBRIDGE
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530084, 31270224] ; Program of Introducing Talents of Discipline to Universities (111 project)Ministry of Education, China - 111 Project [B13007] ; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/R001499/1] Funding Source: UKRI
Corresponding Author Emaillinjx@ibcas.ac.cn
OAGreen Accepted, Bronze
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Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
4.Yale Univ, Sch Med, Dept Cell Biol, 333 Cedar St, New Haven, CT 06520 USA
5.Univ Bonn, Inst Cellular & Mol Bot, D-53115 Bonn, Germany
6.Christie, John M.] MRC, Inst Mol Cell & Syst Biol, Bower Bldg, Glasgow G12 8QQ, Lanark, Scotland
7.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Xue, Yiqun,Xing, Jingjing,Wan, Yinglang,et al. Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains[J]. MOLECULAR PLANT,2018,11(6):846-859.
APA Xue, Yiqun.,Xing, Jingjing.,Wan, Yinglang.,Lv, Xueqin.,Fan, Lusheng.,...&Lin, Jinxing.(2018).Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains.MOLECULAR PLANT,11(6),846-859.
MLA Xue, Yiqun,et al."Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains".MOLECULAR PLANT 11.6(2018):846-859.
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