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| Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance | |
| Zhang, Haiyan1; Yu, Pengli1; Zhao, Jiuhai2; Jiang, Hongling2; Wang, Haiyang3; Zhu, Yingfang4; Botella, Miguel A.; Samaj, Jozef6; Li, Chuanyou2; Lin, Jinxing1,7 | |
| 2018 | |
| 发表期刊 | NEW PHYTOLOGIST
 |
| ISSN | 0028-646X |
| 卷号 | 217期号:2页码:799-812 |
| 摘要 | Systemin (SYS), an octadecapeptide hormone processed from a 200-amino-acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whether PS mRNA is transferable and its role in systemic defense responses remain unknown. We created the transgenic tomato PS gene tagged with the green fluorescent protein (PS-GFP) using a shoot-or root-specific promoter, and the constitutive 35S promoter in Arabidopsis. Subcellular localization of PS-/SYS-GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real-time PCR. In Arabidopsis, PS protein can be processed and SYS is secreted. Shoot-/root-specific expression of PS-GFP in Arabidopsis, and grafting experiments, revealed that the PS mRNA moves in a bi-directional manner. We also found that ectopic expression of PS improves Arabidopsis resistance to the necrotrophic fungus Botrytis cinerea, consistent with substantial upregulation of the transcript levels of specific pathogen-responsive genes. Our results provide novel insights into the multifaceted mechanism of SYS signaling transport and its potential application in genetic engineering for increasing pathogen resistance across diverse plant families. |
| 关键词 | Arabidopsis long-distance movement mobile signal pathogen resistance prosystemin systemin tomato |
| 学科领域 | Plant Sciences |
| DOI | 10.1111/nph.14858 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | LONG-DISTANCE TRAFFICKING ; PLANT DEFENSIVE GENES ; RECEPTOR KINASE ; BIOLOGICAL-ACTIVITY ; ELICITOR PEPTIDES ; BOTRYTIS-CINEREA ; TOBACCO PLANTS ; WOUND RESPONSE ; SIGNAL ; IMMUNITY |
| WOS记录号 | WOS:000419324000030 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1469-8137 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470292, 31270313, 90817010] ; National Program of Sustainability I, MEYS, Czech Republic [LO1204] |
| 作者邮箱 | linjx@ibcas.ac.cn |
| 作品OA属性 | Bronze |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/20440 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06411 USA 5.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA 6.Botella, Miguel A.] Univ Malaga, CSIC, Inst Hortofruticultura Subtrop & Mediterranea La, Dept Biol Mol & Bioquim,IHSM UMA, Campus Teatinos, E-29071 Malaga, Spain 7.Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Dept Cell Biol, Olomouc, Czech Republic 8.Beijing Forestry Univ, Coll Biol Sci, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Haiyan,Yu, Pengli,Zhao, Jiuhai,et al. Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance[J]. NEW PHYTOLOGIST,2018,217(2):799-812. |
| APA | Zhang, Haiyan.,Yu, Pengli.,Zhao, Jiuhai.,Jiang, Hongling.,Wang, Haiyang.,...&Lin, Jinxing.(2018).Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance.NEW PHYTOLOGIST,217(2),799-812. |
| MLA | Zhang, Haiyan,et al."Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance".NEW PHYTOLOGIST 217.2(2018):799-812. |
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| 29105094.pdf（2673KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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