Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds

doi:10.1016/j.gene.2018.05.011

	Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds
	Yin, Dan-Dan ; Li, Shan-Shan ; Shu, Qing-Yan ; Gu, Zhao-Yu ; Wu, Qian ; Feng, Cheng-Yong ; Xu, Wen-Zhong ; Wang, Liang-Sheng
	2018
发表期刊	GENE
ISSN	0378-1119
卷号	666 页码:72-82
摘要	MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) act as important molecular regulators in a wide range of biological processes during plant development and seed formation, including oil production. Tree peony seeds contain > 90% unsaturated fatty acids (UFAs) and high proportions of a-linolenic acid (ALA, > 40%). To dissect the non-coding RNAs (ncRNAs) pathway involved in fatty acids synthesis in tree peony seeds, we construct six small RNA libraries and six transcriptome libraries from developing seeds of two cultivars (J and S) containing different content of fatty acid compositions. After deep sequencing the RNA libraries, the ncRNA expression profiles of tree peony seeds in two cultivars were systematically and comparatively analyzed. A total of 318 known and 153 new miRNAs and 22,430 lncRNAs were identified, among which 106 conserved and 9 novel miRNAs and 2785 lncRNAs were differentially expressed between the two cultivars. In addition, potential target genes of the microRNA and lncRNAs were also predicted and annotated. Among them, 9 miRNAs and 39 lncRNAs were predicted to target lipid related genes. Results showed that all of miR414, miR156b, miR2673b, miR7826, novel-m0027-5p, TR24651 vertical bar c0_gl, TR24544 vertical bar c0_g15, and TR27305 vertical bar c0_g1 were up-regulated and expressed at a higher level in high-ALA cultivar J when compared to low-ALA cultivar S, suggesting that these ncRNAs and target genes are possibly involved in different fatty acid synthesis and lipid metabolism through post-transcriptional regulation. These results provide a better understanding of the roles of ncRNAs during fatty acid biosynthesis and metabolism in tree peony seeds.
关键词	miRNA lncRNA alpha-Linolenic acid Target gene Tree peony seed Paeonia
学科领域	Genetics & Heredity
DOI	10.1016/j.gene.2018.05.011
收录类别	SCI
语种	英语
WOS关键词	ALPHA-LINOLENIC ACID ; GAS-CHROMATOGRAPHY ; BRASSICA-NAPUS ; OIL CONTENT ; GENE ; ARABIDOPSIS ; CULTIVARS ; STRESS ; MASS ; BIOGENESIS
WOS记录号	WOS:000445438700009
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-0038
资助机构	Beijing Agricultural Science and Technology Program [Z161100000916015]
作者邮箱	xuwzh@ibeas.ac.cn ; wanglsh@ibeas.ac.cn
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20458
专题	中科院北方资源植物重点实验室
作者单位	1.[Yin, Dan-Dan 2.Li, Shan-Shan 3.Shu, Qing-Yan 4.Gu, Zhao-Yu 5.Wu, Qian 6.Feng, Cheng-Yong 7.Xu, Wen-Zhong 8.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 9.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yin, Dan-Dan,Li, Shan-Shan,Shu, Qing-Yan,et al. Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds[J]. GENE,2018,666:72-82.
APA	Yin, Dan-Dan.,Li, Shan-Shan.,Shu, Qing-Yan.,Gu, Zhao-Yu.,Wu, Qian.,...&Wang, Liang-Sheng.(2018).Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds.GENE,666,72-82.
MLA	Yin, Dan-Dan,et al."Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds".GENE 666(2018):72-82.

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