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Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds
Yin, Dan-Dan; Li, Shan-Shan; Shu, Qing-Yan; Gu, Zhao-Yu; Wu, Qian; Feng, Cheng-Yong; Xu, Wen-Zhong; Wang, Liang-Sheng
Source PublicationGENE
AbstractMicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) act as important molecular regulators in a wide range of biological processes during plant development and seed formation, including oil production. Tree peony seeds contain > 90% unsaturated fatty acids (UFAs) and high proportions of a-linolenic acid (ALA, > 40%). To dissect the non-coding RNAs (ncRNAs) pathway involved in fatty acids synthesis in tree peony seeds, we construct six small RNA libraries and six transcriptome libraries from developing seeds of two cultivars (J and S) containing different content of fatty acid compositions. After deep sequencing the RNA libraries, the ncRNA expression profiles of tree peony seeds in two cultivars were systematically and comparatively analyzed. A total of 318 known and 153 new miRNAs and 22,430 lncRNAs were identified, among which 106 conserved and 9 novel miRNAs and 2785 lncRNAs were differentially expressed between the two cultivars. In addition, potential target genes of the microRNA and lncRNAs were also predicted and annotated. Among them, 9 miRNAs and 39 lncRNAs were predicted to target lipid related genes. Results showed that all of miR414, miR156b, miR2673b, miR7826, novel-m0027-5p, TR24651 vertical bar c0_gl, TR24544 vertical bar c0_g15, and TR27305 vertical bar c0_g1 were up-regulated and expressed at a higher level in high-ALA cultivar J when compared to low-ALA cultivar S, suggesting that these ncRNAs and target genes are possibly involved in different fatty acid synthesis and lipid metabolism through post-transcriptional regulation. These results provide a better understanding of the roles of ncRNAs during fatty acid biosynthesis and metabolism in tree peony seeds.
KeywordmiRNA lncRNA alpha-Linolenic acid Target gene Tree peony seed Paeonia
Subject AreaGenetics & Heredity
Indexed BySCI
WOS IDWOS:000445438700009
Publication PlaceAMSTERDAM
Funding OrganizationBeijing Agricultural Science and Technology Program [Z161100000916015]
Corresponding Author Emailxuwzh@ibeas.ac.cn ; wanglsh@ibeas.ac.cn
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.[Yin, Dan-Dan
2.Li, Shan-Shan
3.Shu, Qing-Yan
4.Gu, Zhao-Yu
5.Wu, Qian
6.Feng, Cheng-Yong
7.Xu, Wen-Zhong
8.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China
9.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yin, Dan-Dan,Li, Shan-Shan,Shu, Qing-Yan,et al. Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds[J]. GENE,2018,666:72-82.
APA Yin, Dan-Dan.,Li, Shan-Shan.,Shu, Qing-Yan.,Gu, Zhao-Yu.,Wu, Qian.,...&Wang, Liang-Sheng.(2018).Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds.GENE,666,72-82.
MLA Yin, Dan-Dan,et al."Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds".GENE 666(2018):72-82.
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