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Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing
Zhang, Yuling; Giuliani, Rita1; Zhang, Youjun2; Zhang, Yang3; Araujo, Wagner Luiz2; Wang, Baichen4; Liu, Peng5; Sun, Qi6; Cousins, Asaph1; Edwards, Gerald1; Fernie, Alisdair2; Brutnell, Thomas P.; Li, Pinghua
2018
发表期刊JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN1672-9072
卷号60期号:8页码:670-690
摘要In C-4 photosynthesis, pyruvate orthophosphate dikinase (PPDK) catalyzes the regeneration of phosphoenolpyruvate in the carbon shuttle pathway. Although the biochemical function of PPDK in maize is well characterized, a genetic analysis of PPDK has not been reported. In this study, we use the maize transposable elements Mutator and Ds to generate multiple mutant alleles of PPDK. Loss-of-function mutants are seedling lethal, even when plants were grown under 2% CO2, and they show very low capacity for CO2 assimilation, indicating C-4 photosynthesis is essential in maize. Using RNA-seq and GC-MS technologies, we examined the transcriptional and metabolic responses to a deficiency in PPDK activity. These results indicate loss of PPDK results in downregulation of gene expression of enzymes of the C-4 cycle, the Calvin cycle, and components of photochemistry. Furthermore, the loss of PPDK did not change Kranz anatomy, indicating that this metabolic defect in the C-4 cycle did not impinge on the morphological differentiation of C-4 characters. However, sugar metabolism and nitrogen utilization were altered in the mutants. An interaction between light intensity and genotype was also detected from transcriptome profiling, suggesting altered transcriptional and metabolic responses to environmental and endogenous signals in the PPDK mutants.
学科领域Biochemistry & Molecular Biology ; Plant Sciences
DOI10.1111/jipb.12656
收录类别SCI
语种英语
WOS关键词NADP-MALATE DEHYDROGENASE ; BUNDLE-SHEATH-CELLS ; C-4 PHOTOSYNTHESIS ; PYRUVATE,ORTHOPHOSPHATE DIKINASE ; DIFFERENTIAL EXPRESSION ; NITROGEN REMOBILIZATION ; GAS-CHROMATOGRAPHY ; GLOBAL FOOD ; ZEA-MAYS ; ARABIDOPSIS
WOS记录号WOS:000440682400004
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1744-7909
资助机构NSFCNational Natural Science Foundation of China (NSFC) [31271393] ; National Key Research and Developmen Program of China [2016YFD0101003] ; Taishan Program ; NSFNational Science Foundation (NSF) [EF-1105249, IOS-092270, IOS-1127017]
作者邮箱pinghuali@sdau.edu.cn
作品OA属性Bronze
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/20474
专题中科院光生物学重点实验室
作者单位1.Shandong Agr Univ, Coll Agron Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Washington State Univ, Sch Biol Sci, Mol Plant Sci, Pullman, WA 99164 USA
3.Max Planck Inst Mol Pflanzenphysiol, Muhlenberg 1, D-14476 Potsdam, Germany
4.Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA
5.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China
6.Iowa State Univ, Dept Stat, Ames, IA 50011 USA
7.Cornell Univ, Life Sci Core Labs Ctr, Computat Biol Serv Unit, Ithaca, NY 14850 USA
8.Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA
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Zhang, Yuling,Giuliani, Rita,Zhang, Youjun,et al. Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2018,60(8):670-690.
APA Zhang, Yuling.,Giuliani, Rita.,Zhang, Youjun.,Zhang, Yang.,Araujo, Wagner Luiz.,...&Li, Pinghua.(2018).Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,60(8),670-690.
MLA Zhang, Yuling,et al."Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 60.8(2018):670-690.
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