Changes in the mitochondrial proteome of developing maize seed embryos

doi:10.1111/ppl.12725

	Changes in the mitochondrial proteome of developing maize seed embryos
	Wang, Wei-Qing ; Wang, Yue ; Zhang, Qi 4; Moller, Ian M.; Song, Song-Quan
	2018
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	163 期号:4 页码:552-572
摘要	Mitochondria are required for seed development, but little information is available about their function and role during this process. We isolated the mitochondria from developing maize (Zea mays L. cv. Nongda 108) embryos and investigated the mitochondrial membrane integrity and respiration as well as the mitochondrial proteome using two proteomic methods, the two-dimensional gel electrophoresis (2-DE) and sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH). Mitochondrial membrane integrity and respiration were maintained at a high level up to 21 days after pollination (DAP) and decreased thereafter, while total mitochondrial number, cytochrome c oxidase activity and respiration per embryo exhibited a bell-shaped change with peaks at 35-45 DAP. A total of 286 mitochondrial proteins changed in abundance during embryo development. During early stages of seed development (up to 21 DAP), proteins involved in energy production, basic metabolism, protein import and folding as well as removal of reactive oxygen species dominated, while during mid or late stages (35-70 DAP), some stress- and detoxification-related proteins increased in abundance. Our study, for the first time, depicted a relatively comprehensive map of energy production by mitochondria during embryo development. The results revealed that mitochondria were very active during the early stages of maize embryo development, while at the late stages of development, the mitochondria became more quiescent, but well-protected, presumably to ensure that the embryo passes through maturation, drying and long-term storage. These results advance our understanding of seed development at the organelle level.
学科领域	Plant Sciences
DOI	10.1111/ppl.12725
收录类别	SCI
语种	英语
WOS关键词	DESICCATION TOLERANCE ; ELECTRON-TRANSPORT ; PLANT-MITOCHONDRIA ; SUBCELLULAR-LOCALIZATION ; QUANTITATIVE PROTEOMICS ; PROTEINS ; ARABIDOPSIS ; STRESS ; GERMINATION ; BIOGENESIS
WOS记录号	WOS:000440004000009
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1399-3054
资助机构	National Sciences and Technology Support Program [2012BAC01B05] ; National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400292] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Sciences Visiting Professorship for senior international scientists
作者邮箱	ian.max.moller@mbg.au.dk ; sqsong@ibcas.ac.cn
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20497
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Moller, Ian M.] Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark 5.Guangdong Acad Agr Sci, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wei-Qing,Wang, Yue,Zhang, Qi,et al. Changes in the mitochondrial proteome of developing maize seed embryos[J]. PHYSIOLOGIA PLANTARUM,2018,163(4):552-572.
APA	Wang, Wei-Qing,Wang, Yue,Zhang, Qi,Moller, Ian M.,&Song, Song-Quan.(2018).Changes in the mitochondrial proteome of developing maize seed embryos.PHYSIOLOGIA PLANTARUM,163(4),552-572.
MLA	Wang, Wei-Qing,et al."Changes in the mitochondrial proteome of developing maize seed embryos".PHYSIOLOGIA PLANTARUM 163.4(2018):552-572.

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