Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana

doi:10.1007/s11103-018-0795-9

	Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana
	Gong, Pichang ; Li, Jing 1; He, Chaoying1
	2018
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	98 期号:6 页码:545-563
摘要	Key message Molecular and functional characterization of four gene families of the Physalis exon junction complex (EJC) core improved our understanding of the evolution and function of EJC core genes in plants. Abstract The exon junction complex (EJC) plays significant roles in posttranscriptional regulation of genes in eukaryotes. However, its developmental roles in plants are poorly known. We characterized four EJC core genes from Physalis floridana that were named PFMAGO, PFY14, PFeIF4AIII and PFBTZ. They shared a similar phylogenetic topology and were expressed in all examined organs. PFMAGO, PFY14 and PFeIF4AIII were localized in both the nucleus and cytoplasm while PFBTZ was mainly localized in the cytoplasm. No protein homodimerization was observed, but they could form heterodimers excluding the PFY14-PFBTZ heterodimerization. Virus-induced gene silencing (VIGS) of PFMAGO or PFY14 aborted pollen development and resulted in low plant survival due to a leaf-blight-like phenotype in the shoot apex. Carpel functionality was also impaired in the PFY14 knockdowns, whereas pollen maturation was uniquely affected in PFBTZ-VIGS plants. Once PFeIF4AIII was strongly downregulated, plant survival was reduced via a decomposing root collar after flowering and Chinese lantern morphology was distorted. The expression of Physalis orthologous genes in the DYT1-TDF1-AMS-bHLH91 regulatory cascade that is associated with pollen maturation was significantly downregulated in PFMAGO-, PFY14- and PFBTZ-VIGS flowers. Intron-retention in the transcripts of P. floridana dysfunctional tapetum1 (PFDYT1) occurred in these mutated flowers. Additionally, the expression level of WRKY genes in defense-related pathways in the shoot apex of PFMAGO- or PFY14-VIGS plants and in the root collar of PFeIF4AIII-VIGS plants was significantly downregulated. Taken together, the Physalis EJC core genes play multiple roles including a conserved role in male fertility and newly discovered roles in Chinese lantern development, carpel functionality and defense-related processes. These data increase our understanding of the evolution and functions of EJC core genes in plants.
关键词	Pre-mRNA splicing Defense-related process Exon junction complex (EJC) Functional evolution Pollen maturation Physalis floridana
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-018-0795-9
收录类别	SCI
语种	英语
WOS关键词	OSKAR MESSENGER-RNA ; MAGO-NASHI ; TRANSCRIPTION FACTOR ; MOLECULAR CHARACTERIZATION ; EXPRESSION ANALYSIS ; ANTHER DEVELOPMENT ; CRYSTAL-STRUCTURE ; CAP-BINDING ; PROTEIN ; INTERACTS
WOS记录号	WOS:000452327100005
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370259, 31525003]
作者邮箱	chaoying@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20498
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gong, Pichang,Li, Jing,He, Chaoying. Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana[J]. PLANT MOLECULAR BIOLOGY,2018,98(6):545-563.
APA	Gong, Pichang,Li, Jing,&He, Chaoying.(2018).Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana.PLANT MOLECULAR BIOLOGY,98(6),545-563.
MLA	Gong, Pichang,et al."Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana".PLANT MOLECULAR BIOLOGY 98.6(2018):545-563.

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