A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm

doi:10.1093/jxb/ery290

	A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm
	Tian, Lihong ; Xing, Yanping ; Fukuda, Masako 1; Li, Rong ; Kumamaru, Toshihiro 1; Qian, Dandan ; Dong, Xiangbai ; Qu, Le Qing
	2018
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	69 期号:21 页码:5029-5043
摘要	Rice glutelins are initially synthesized as 57-kDa precursors at the endoplasmic reticulum (ER) and are ultimately transported into protein storage vacuoles. However, the sequence motifs that affect proglutelin folding, assembly, and their export from the ER remain poorly defined. In this study, we characterized a mutant with nine amino acids deleted in the GluA2 protein, which resulted in specific accumulation of the GluA precursor. The deleted amino acids constitute a well-conserved sequence (LVYIIQGRG) in glutelins and all residues in this motif are necessary for ER export of GluA2. Immunoelectron microscopy and stable transgenic analyses indicated that proglutelins with deletion of this motif misassembled and aggregated through non-native intermolecular disulfide bonds, and were deposited in ER-derived protein bodies (PB-Is), resulting in conversion of PB-Is into a new type of PB. These results indicate that the conserved motif is essential for proper assembly of proglutelin. The correct assembly of proglutelins is critical for their segregation from prolamins in the ER lumen, which is essential for enabling the export of proglutelin from the ER and for the proper formation of PB-Is. We also found that the interchain disulfide bond between acidic and basic subunits is not necessary for their assembly, but it is required for proglutelin folding.
关键词	Disulfide bonds endoplasmic reticulum export glutelin rice endosperm sequence motif protein assembly
学科领域	Plant Sciences
DOI	10.1093/jxb/ery290
收录类别	SCI
语种	英语
WOS关键词	PROTEIN STORAGE VACUOLE ; DISULFIDE BOND FORMATION ; INTRACELLULAR-TRANSPORT ; STARCHY ENDOSPERM ; GOLGI-APPARATUS ; ARABIDOPSIS-THALIANA ; GLUTELIN PRECURSOR ; RAB5 PROTEINS ; EXPRESSION ; ISOMERASE
WOS记录号	WOS:000448163900005
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD 0100500] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300212, 31171368]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20507
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Kyushu Univ, Fac Agr, Fukuoka, Fukuoka 8128581, Japan
推荐引用方式 GB/T 7714	Tian, Lihong,Xing, Yanping,Fukuda, Masako,et al. A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(21):5029-5043.
APA	Tian, Lihong.,Xing, Yanping.,Fukuda, Masako.,Li, Rong.,Kumamaru, Toshihiro.,...&Qu, Le Qing.(2018).A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm.JOURNAL OF EXPERIMENTAL BOTANY,69(21),5029-5043.
MLA	Tian, Lihong,et al."A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm".JOURNAL OF EXPERIMENTAL BOTANY 69.21(2018):5029-5043.

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