IB-CAS  > 中科院植物分子生理学重点实验室
A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm
Tian, Lihong; Xing, Yanping; Fukuda, Masako1; Li, Rong; Kumamaru, Toshihiro1; Qian, Dandan; Dong, Xiangbai; Qu, Le Qing
2018
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
Volume69Issue:21Pages:5029-5043
AbstractRice glutelins are initially synthesized as 57-kDa precursors at the endoplasmic reticulum (ER) and are ultimately transported into protein storage vacuoles. However, the sequence motifs that affect proglutelin folding, assembly, and their export from the ER remain poorly defined. In this study, we characterized a mutant with nine amino acids deleted in the GluA2 protein, which resulted in specific accumulation of the GluA precursor. The deleted amino acids constitute a well-conserved sequence (LVYIIQGRG) in glutelins and all residues in this motif are necessary for ER export of GluA2. Immunoelectron microscopy and stable transgenic analyses indicated that proglutelins with deletion of this motif misassembled and aggregated through non-native intermolecular disulfide bonds, and were deposited in ER-derived protein bodies (PB-Is), resulting in conversion of PB-Is into a new type of PB. These results indicate that the conserved motif is essential for proper assembly of proglutelin. The correct assembly of proglutelins is critical for their segregation from prolamins in the ER lumen, which is essential for enabling the export of proglutelin from the ER and for the proper formation of PB-Is. We also found that the interchain disulfide bond between acidic and basic subunits is not necessary for their assembly, but it is required for proglutelin folding.
KeywordDisulfide bonds endoplasmic reticulum export glutelin rice endosperm sequence motif protein assembly
Subject AreaPlant Sciences
DOI10.1093/jxb/ery290
Indexed BySCI
Language英语
WOS KeywordPROTEIN STORAGE VACUOLE ; DISULFIDE BOND FORMATION ; INTRACELLULAR-TRANSPORT ; STARCHY ENDOSPERM ; GOLGI-APPARATUS ; ARABIDOPSIS-THALIANA ; GLUTELIN PRECURSOR ; RAB5 PROTEINS ; EXPRESSION ; ISOMERASE
WOS IDWOS:000448163900005
PublisherOXFORD UNIV PRESS
SubtypeArticle
Publication PlaceOXFORD
EISSN1460-2431
Funding OrganizationMinistry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD 0100500] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300212, 31171368]
Corresponding Author Emaillqqu@ibcas.ac.cn
OAGreen Published, hybrid
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/20507
Collection中科院植物分子生理学重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Kyushu Univ, Fac Agr, Fukuoka, Fukuoka 8128581, Japan
Recommended Citation
GB/T 7714
Tian, Lihong,Xing, Yanping,Fukuda, Masako,et al. A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(21):5029-5043.
APA Tian, Lihong.,Xing, Yanping.,Fukuda, Masako.,Li, Rong.,Kumamaru, Toshihiro.,...&Qu, Le Qing.(2018).A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm.JOURNAL OF EXPERIMENTAL BOTANY,69(21),5029-5043.
MLA Tian, Lihong,et al."A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm".JOURNAL OF EXPERIMENTAL BOTANY 69.21(2018):5029-5043.
Files in This Item:
File Name/Size DocType Version Access License
Tian-2018-A conserve(7811KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tian, Lihong]'s Articles
[Xing, Yanping]'s Articles
[Fukuda, Masako]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tian, Lihong]'s Articles
[Xing, Yanping]'s Articles
[Fukuda, Masako]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tian, Lihong]'s Articles
[Xing, Yanping]'s Articles
[Fukuda, Masako]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Tian-2018-A conserved motif is essential for t.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.