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| Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms | |
Ji, Dongchao1; Chen, Tong; Ma, Danying1; Liu, Jialei2; Xu, Yong; Tian, Shiping1,3
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| 2018 | |
| Source Publication | POSTHARVEST BIOLOGY AND TECHNOLOGY
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| ISSN | 0925-5214 |
| Volume | 142Pages:46-54 |
| Abstract | Methyl thujate, a monoterpenoid substance, was effective in controlling postharvest gray mold caused by Botrytis cinerea on apple fruit, during application in aqueous solution. Methyl thujate strongly inhibited spore germination, germ tube elongation and mycelial spreading of B. cinerea in the culture medium, and the inhibition on fruit decay was closely correlated with methyl thujate concentrations. By means of propidium iodide and fluorescein diacetate staining, loss of membrane integrity and cell viability in B. cinerea was detected after methyl thujate treatment. Furthermore, methyl thujate led to cytoplasmic accumulation of reactive oxygen species, leakage of cellular constituents and variations in cell wall component deposition in B. cinerea hyphae, as the genes encoded subunits for NADPH oxidase were synergistically upregulated, whereas the genes involved in cell wall modeling and virulence responded differentially. Taken together, these data suggest that the mechanisms by which methyl thujate decreased fruit decay may be directly attributed to the induced ROS burst, breakdown of cell membrane integrity and loss of cytoplasmic contents from the hyphae. |
| Keyword | Botrytis cinerea Methyl thujate Reactive oxygen species Membrane integrity |
| Subject Area | Agronomy ; Food Science & Technology ; Horticulture |
| DOI | 10.1016/j.postharvbio.2018.04.003 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PENICILLIUM-EXPANSUM ; GENE-EXPRESSION ; NADPH OXIDASE ; GRAY MOLD ; IN-VITRO ; VIRULENCE ; PATHOGENICITY ; COMPONENT ; SUBUNIT ; DISEASE |
| WOS ID | WOS:000430885400006 |
| Publisher | ELSEVIER |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 1873-2356 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 30672210] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3, 2016YFD0400903, 2017YFD0401301] |
| Corresponding Author Email | tsp@ibcas.ac.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/20515 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Phys & Chem Tech, Beijing 100093, Peoples R China 4.Minist Agr, Key Lab Postharvest Handling Fruits, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Ji, Dongchao,Chen, Tong,Ma, Danying,et al. Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2018,142:46-54. |
| APA | Ji, Dongchao,Chen, Tong,Ma, Danying,Liu, Jialei,Xu, Yong,&Tian, Shiping.(2018).Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms.POSTHARVEST BIOLOGY AND TECHNOLOGY,142,46-54. |
| MLA | Ji, Dongchao,et al."Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms".POSTHARVEST BIOLOGY AND TECHNOLOGY 142(2018):46-54. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| j.postharvbio.2018.0（2653KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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