IB-CAS  > 中科院北方资源植物重点实验室
Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms
Ji, Dongchao1; Chen, Tong; Ma, Danying1; Liu, Jialei2; Xu, Yong; Tian, Shiping1,3
2018
Source PublicationPOSTHARVEST BIOLOGY AND TECHNOLOGY
ISSN0925-5214
Volume142Pages:46-54
AbstractMethyl thujate, a monoterpenoid substance, was effective in controlling postharvest gray mold caused by Botrytis cinerea on apple fruit, during application in aqueous solution. Methyl thujate strongly inhibited spore germination, germ tube elongation and mycelial spreading of B. cinerea in the culture medium, and the inhibition on fruit decay was closely correlated with methyl thujate concentrations. By means of propidium iodide and fluorescein diacetate staining, loss of membrane integrity and cell viability in B. cinerea was detected after methyl thujate treatment. Furthermore, methyl thujate led to cytoplasmic accumulation of reactive oxygen species, leakage of cellular constituents and variations in cell wall component deposition in B. cinerea hyphae, as the genes encoded subunits for NADPH oxidase were synergistically upregulated, whereas the genes involved in cell wall modeling and virulence responded differentially. Taken together, these data suggest that the mechanisms by which methyl thujate decreased fruit decay may be directly attributed to the induced ROS burst, breakdown of cell membrane integrity and loss of cytoplasmic contents from the hyphae.
KeywordBotrytis cinerea Methyl thujate Reactive oxygen species Membrane integrity
Subject AreaAgronomy ; Food Science & Technology ; Horticulture
DOI10.1016/j.postharvbio.2018.04.003
Indexed BySCI
Language英语
WOS KeywordPENICILLIUM-EXPANSUM ; GENE-EXPRESSION ; NADPH OXIDASE ; GRAY MOLD ; IN-VITRO ; VIRULENCE ; PATHOGENICITY ; COMPONENT ; SUBUNIT ; DISEASE
WOS IDWOS:000430885400006
PublisherELSEVIER
SubtypeArticle
Publication PlaceAMSTERDAM
EISSN1873-2356
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 30672210] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3, 2016YFD0400903, 2017YFD0401301]
Corresponding Author Emailtsp@ibcas.ac.cn
Citation statistics
Cited Times:53[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/20515
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Phys & Chem Tech, Beijing 100093, Peoples R China
4.Minist Agr, Key Lab Postharvest Handling Fruits, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Ji, Dongchao,Chen, Tong,Ma, Danying,et al. Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2018,142:46-54.
APA Ji, Dongchao,Chen, Tong,Ma, Danying,Liu, Jialei,Xu, Yong,&Tian, Shiping.(2018).Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms.POSTHARVEST BIOLOGY AND TECHNOLOGY,142,46-54.
MLA Ji, Dongchao,et al."Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms".POSTHARVEST BIOLOGY AND TECHNOLOGY 142(2018):46-54.
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