The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum

doi:10.1111/1462-2920.14453

	The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum
	Chen, Yong 1; Li, Boqiang ; Xu, Xiaodi 1; Zhang, Zhanquan ; Tian, Shiping1,2
	2018
发表期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
卷号	20 期号:11 页码:4063-4078
摘要	The PacC (loss or reduction in phosphatase activity at acid but not at alkaline pH [Pac]) transcription factor regulates environmental adaptation, secondary metabolism and virulence in many fungal pathogens. Here, we report the functions of PacC in Penicillium expansum, a postharvest pathogenic fungus in horticultural crops, and ascertain that the gene expression and proteolytic processing of PePacC are strictly pH-dependent. Loss of PePacC resulted in an obvious decrease in growth and conidiation of P. expansum cultured in both acidic and alkaline conditions. The Delta PePacC mutant lost the ability of patulin production at pH values above 6.0 because expressions of all the genes in patulin cluster were significantly down-regulated. Additionally, virulence of the Delta PePacC mutant was obviously reduced in pear and apple fruits. Proteome analysis revealed that PePacC could function as an activator or repressor for different target proteins, including calreticulin (PeCRT) and sulfate adenylyltransferase (PeSAT), which were further proved to be involved in virulence of P. expansum. Our results demonstrate important roles for PePacC in patulin biosynthesis via limiting expressions of the genes in the cluster, and in pathogenesis via mediating a known virulence factor glucose oxidase (PeGOD) and new virulence factors, such as PeCRT and PeSAT.
学科领域	Microbiology
DOI	10.1111/1462-2920.14453
收录类别	SCI
语种	英语
WOS关键词	AMBIENT PH ; GENE-EXPRESSION ; MOLECULAR CHARACTERIZATION ; REGULATORY GENE ; PATHOGENICITY ; APPLE ; ACIDIFICATION ; COLONIZATION ; ACCUMULATION ; INVOLVEMENT
WOS记录号	WOS:000450357400013
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1462-2920
资助机构	National Key R&D Program of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31722043]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：63[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20532
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Educ, Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yong,Li, Boqiang,Xu, Xiaodi,et al. The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum[J]. ENVIRONMENTAL MICROBIOLOGY,2018,20(11):4063-4078.
APA	Chen, Yong,Li, Boqiang,Xu, Xiaodi,Zhang, Zhanquan,&Tian, Shiping.(2018).The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum.ENVIRONMENTAL MICROBIOLOGY,20(11),4063-4078.
MLA	Chen, Yong,et al."The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum".ENVIRONMENTAL MICROBIOLOGY 20.11(2018):4063-4078.

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