OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress

doi:10.1104/pp.18.00375

	OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress
	Qian, Dandan; Chen, Guoqiang; Tian, Lihong; Qu, Le Qing
	2018
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	178 期号:1 页码:402-412
摘要	Endoplasmic reticulum-associated protein degradation (ERAD) plays an important role in endoplasmic reticulum (ER) quality control. To date, little is known about the retrotranslocation machinery in the plant ERAD pathway. We obtained a DERLIN-like protein (OsDER1) through a SWATH-based quantitative proteomic analysis of ER membrane proteins extracted from ER-stressed rice (Oryza sativa) seeds. OsDER1, a homolog of yeast and mammal DER1, is localized in the ER and accumulates significantly under ER stress. Overexpression or suppression of OsDER1 in rice leads to activation of the unfolded protein response and hypersensitivity to ER stress, and suppression results in floury, shrunken seeds. In addition, the expression levels of polyubiquitinated proteins increased markedly in OsDER1 overexpression or suppression transgenic rice. Coimmunoprecipitation experiments demonstrated that OsDER1 interacted with OsHRD1, OsHRD3, and OsCDC48, the essential components of the canonical ERAD pathway. Furthermore, OsDER1 associated with the signal peptide peptidase, a homolog of a component of the alternative ERAD pathway identified recently in yeast and mammals. Our data suggest that OsDER1 is linked to the ERAD pathway.
学科领域	Plant Sciences
DOI	10.1104/pp.18.00375
收录类别	SCI
语种	英语
WOS关键词	ENDOPLASMIC-RETICULUM STRESS ; MEMBRANE-PROTEIN ; RICE ENDOSPERM ; UBIQUITIN LIGASE ; QUALITY-CONTROL ; MISFOLDED GLYCOPROTEINS ; STORAGE PROTEINS ; GENE-EXPRESSION ; COMPLEX ; ARABIDOPSIS
WOS记录号	WOS:000444004100031
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0100500] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770271, 31171368, 31300212]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20539
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qian, Dandan,Chen, Guoqiang,Tian, Lihong,et al. OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress[J]. PLANT PHYSIOLOGY,2018,178(1):402-412.
APA	Qian, Dandan,Chen, Guoqiang,Tian, Lihong,&Qu, Le Qing.(2018).OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress.PLANT PHYSIOLOGY,178(1),402-412.
MLA	Qian, Dandan,et al."OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress".PLANT PHYSIOLOGY 178.1(2018):402-412.

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