ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

doi:10.1093/jxb/ery139

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	ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings
	Jiang, Tian 1; Zhang, Jing 1; Rong, Liwei 1; Feng, Yanjiang 2; Wang, Qi 2; Song, Qiulai 2; Zhang, Lixin1 ; Ouyang, Min
	2018
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	69 期号:12 页码:3037-3051
摘要	Chloroplast development is a highly complex process and the regulatory mechanisms have not yet been fully characterized. In this study, we identified Early Chloroplast Development 1 (ECD1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 in Arabidopsis led to embryo lethality, and abnormal embryogenesis occurred in ecdl/+ heterozygous plants. A decrease in ECD1 expression induced by RNAi resulted in seedlings with albino cotyledons but normal true leaves. The aberrant morphology and under-developed thylakoid membrane system in cotyledons of RNAi seedlings suggests a role of ECD1 specifically in chloroplast development in seedlings. In cotyledons of ECD1-RNAi plants, RNA-editing of rps14-149 (encoding ribosomal protein S14) was seriously impaired. In addition, dramatically decreased plastid-encoded RNA polymerase-dependent gene expression and abnormal chloroplast rRNA processing were also observed. Taken together, our results indicate that ECD1 is indispensable for chloroplast development at the seedling stage in Arabidopsis.
关键词	Arabidopsis chloroplast development cotyledon Early Chloroplast Development 1 (ECD1) early stages pentatricopeptide repeat protein (PPR) RNA editing
学科领域	Plant Sciences
DOI	10.1093/jxb/ery139
收录类别	SCI
语种	英语
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEINS ; RIBOSOME-DEFICIENT PLASTIDS ; GENE-EXPRESSION ; ARABIDOPSIS-THALIANA ; PPR PROTEIN ; DIFFERENTIAL REGULATION ; EMBRYO DEVELOPMENT ; PLANT DEVELOPMENT ; ESCHERICHIA-COLI ; MESSENGER-RNA
WOS记录号	WOS:000434047800015
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150100] ; Youth Innovation Promotion Association CAS [2013059]
作者邮箱	ouyangmin@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20573
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Heilongjiang Acad Agr Sci, Cultivat & Crop Tillage Inst, Harbin 150086, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Tian,Zhang, Jing,Rong, Liwei,et al. ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(12):3037-3051.
APA	Jiang, Tian.,Zhang, Jing.,Rong, Liwei.,Feng, Yanjiang.,Wang, Qi.,...&Ouyang, Min.(2018).ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings.JOURNAL OF EXPERIMENTAL BOTANY,69(12),3037-3051.
MLA	Jiang, Tian,et al."ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings".JOURNAL OF EXPERIMENTAL BOTANY 69.12(2018):3037-3051.

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