Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii

doi:10.1016/j.envexpbot.2018.09.017

	Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii
	Li, Jing-Yi ; Li, Dong ; Du, Xue 1; Li, Hui ; Wang, Di ; Xing, Quan ; Yao, Ran 2; Sun, Mei-Yu ; Shi, Lei
	2018
发表期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
卷号	156 页码:298-315
摘要	Drynaria roosii is a traditional medicinal fern, whose rhizome is particularly valued for its effective flavonoid components of naringin/neoeriocitrin. UV irradiation can induce flavonoid accumulation in plants, however the molecular mechanism of UVC-mediated naringin/neoeriocitrin synthesis in D. roosii has never been reported. Our HPLC-tandem mass spectrometry (MS/MS) results showed that UVC irradiation increased naringin/neoeriocitrin contents (NNCs) based on irradiation dosage in D. roosii. According to the NGS data, DEG-based heat map analysis revealed up-regulation of naringin/neoeriocitrin synthetic genes was more obvious in 12h UVC irradiation (UV dose = 81.648 kJ m(-2)) when compared with 24h (UV dose = 163.296 kJ m(-2)), especially for PAL, C3'H and HCT in old rhizomes. Through systems biology method of modular organization analysis, we clustered 15,678 DEGs into 19 modules, and calculated correlation coefficients between modules and samples, as well as modules and NNCs. Four significant naringin-related modules were achieved and displayed high correlations with specific samples. Moreover, weighted gene co-expression network analysis results discovered that 4CL, CHS and HCT, C3H acted as the hub responsive genes to UVC irradiation involved in naringin and neoeriocitrin synthesis respectively, and presented high co-expression with MYB/bHLH-regulated DEGs. Overall, we demonstrated that UVC irradiation up-regulated the naringin/neoeriocitrin related gene expression in separate new and old rhizomes to enhance NNCs in a dose-dependent manner. Hormetic UVC dose ranges improving NNCs in D. roosii were established for the first time. Our work also provided new insights into the study of secondary metabolites in medicinal plants.
关键词	Drynaria roosii Naringin/neoeriocitrin synthesis Modular organization analysis UVC irradiation WGCNA
学科领域	Plant Sciences ; Environmental Sciences
DOI	10.1016/j.envexpbot.2018.09.017
收录类别	SCI
语种	英语
WOS关键词	FLAVONOID BIOSYNTHESIS ; ANTIOXIDANT CAPACITY ; TRANSCRIPTION FACTOR ; ARABIDOPSIS ; NETWORK ; LIGHT ; IDENTIFICATION ; ACCUMULATION ; METABOLOME ; INDUCTION
WOS记录号	WOS:000448093600029
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7307
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573519, 31600264] ; Key Projects of the Chinese Academy of Sciences [KZCC-EW-103-3]
作者邮箱	jingyileesd@126.com ; lidongfern@126.com ; snowingdu@163.com ; lihuibjfu@126.com ; wangdi06060@163.com ; xq616@126.com ; yaoran@semi.ac.cn ; sunmeiyu@ibcas.ac.cn ; shilei_67@126.com
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20574
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Semicond, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing-Yi,Li, Dong,Du, Xue,et al. Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2018,156:298-315.
APA	Li, Jing-Yi.,Li, Dong.,Du, Xue.,Li, Hui.,Wang, Di.,...&Shi, Lei.(2018).Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,156,298-315.
MLA	Li, Jing-Yi,et al."Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 156(2018):298-315.

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