Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation

doi:10.1016/j.plantsci.2018.05.027

	Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation
	Yin, Dan-Dan ; Xu, Wen-Zhong ; Shu, Qing-Yan ; Li, Shan-Shan ; Wu, Qian ; Feng, Cheng-Yong ; Gu, Zhao-Yu ; Wang, Liang-Sheng
	2018
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	274 页码:212-222
摘要	alpha-linolenic acid (ALA) deficiency and a skewed m6: co3 fatty acid ratio in the diet are thought to be a major cause for the high incidence of cardiovascular, inflammatory, and autoimmune diseases. Recent years, tree peony (Paeonia suffruticosa Andr.) with the high proportion of ALA (more than 45% in seed oil) is widely concerned. However, the underlying accumulation mechanism of the ALA in tree peony seeds remains unknown. In this study, comparative transcriptome analysis was performed between two cultivars ('Saiguifer and 'Jingshenhuanfa') with different ALA contents. The analysis of the metabolic enzymes associated with ALA biosynthesis and temporal accumulation patterns of unsaturated fatty acids demonstrated the importance of microsomal omega-3 fatty acid desaturase 3 (FAD3). Moreover, PsFAD3 gene was identified from tree peony seeds, which was located in endoplasmic reticulum and the expression levels of PsFAD3 were consistent with ALA accumulation patterns in seeds. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated PsFAD3 protein could catalyze ALA synthesis. These results indicated that PsFAD3 was involved in the synthesis of ALA in seeds and could be exploited by the genetic breeding of new cultivars with high ALA content in tree peony as well as other potential crops.
关键词	alpha-Linolenic acid Comparative transcriptome Fatty acid desaturase Paeoniaceae Tree peony
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.plantsci.2018.05.027
收录类别	SCI
语种	英语
WOS关键词	SACCHAROMYCES-CEREVISIAE ; MOLECULAR-CLONING ; RNA-SEQ ; GENES ; EXPRESSION ; IDENTIFICATION ; ARABIDOPSIS ; SEED ; TRANSCRIPTOME ; BIOSYNTHESIS
WOS记录号	WOS:000442068200024
出版者	ELSEVIER IRELAND LTD
文献子类	Article
出版地	CLARE
资助机构	Beijing Agricultural Science and Technology Program [Z161100000916015]
作者邮箱	shshli@ibcas.ac.cn ; wanglsh@ibcas.ac.cn
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20627
专题	中科院北方资源植物重点实验室
作者单位	1.[Yin, Dan-Dan 2.Xu, Wen-Zhong 3.Shu, Qing-Yan 4.Li, Shan-Shan 5.Wu, Qian 6.Feng, Cheng-Yong 7.Gu, Zhao-Yu 8.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 9.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yin, Dan-Dan,Xu, Wen-Zhong,Shu, Qing-Yan,et al. Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation[J]. PLANT SCIENCE,2018,274:212-222.
APA	Yin, Dan-Dan.,Xu, Wen-Zhong.,Shu, Qing-Yan.,Li, Shan-Shan.,Wu, Qian.,...&Wang, Liang-Sheng.(2018).Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation.PLANT SCIENCE,274,212-222.
MLA	Yin, Dan-Dan,et al."Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation".PLANT SCIENCE 274(2018):212-222.

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