Knowledge Management System Of Institute Of Botany,CAS
| CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis | |
| Jiang, Xiaolan; Huang, Keyi1; Zheng, Guangshun2,3,4; Hou, Hua1; Wang, Peiqiang; Jiang, Han; Zhao, Xuecheng1; Li, Mingzhuo; Zhang, Shuxiang1; Liu, Yajun1; Gao, Liping1; Zhao, Lei5; Xia, Tao | |
| 2018 | |
| 发表期刊 | PLANT SCIENCE
 |
| ISSN | 0168-9452 |
| 卷号 | 270页码:209-220 |
| 摘要 | Tea is one of the most widely consumed nonalcoholic beverages worldwide. Polyphenols are nutritional compounds present in the leaves of tea plants. Although numerous genes are functionally characterized to encode enzymes that catalyze the formation of diverse polyphenolic metabolites, transcriptional regulation of those different pathways such as late steps of the proanthcoyanidin (PA) pathway remains unclear. In this study, using different tea transcriptome databases, we screened at least 140 R2R3-MYB transcription factors (TFs) and grouped them according to the basic function domains of the R2R3 MYB TF superfamily. Among 140 R2R3 TFs, CsMYB5a and CsMYB5e were chosen for analysis because they may be involved in PA biosynthesis regulation. CsMYB5a-overexpressing tobacco plants exhibited downregulated anthocyanin accumulation but a high polymeric PA content in the flowers. Overexpression of CsMYB5e in tobacco plants did not change the anthocyanin content but increased the dimethylaminocinnamaldehyde-stained PA content. RNA-seq and qRT-PCR analyses revealed that genes related to PA and anthocyanin biosynthesis pathways were markedly upregulated in both CsMYB5a- and CsMYB5e-overexpressing flowers. Three UGTs and four GSTs were identified as involved in PA and anthocyanin glycosylation and transportation in transgenic plants. These results provide new insights into the regulation of PA and anthocyanin biosynthesis in Camellia sinensis. |
| 关键词 | Camellia sinensis Proanthocyanidin Anthocyanin CsMYB5a and CsMYB5e Polygenic regulatory mechanism |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.plantsci.2018.02.009 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | MYB TRANSCRIPTION FACTOR ; SEED COAT ; FUNCTIONAL-CHARACTERIZATION ; LOW-TEMPERATURE ; GENE ENCODES ; EXPRESSION ; GRAPEVINE ; REDUCTASE ; TT2 ; ACCUMULATION |
| WOS记录号 | WOS:000430777600021 |
| 出版者 | ELSEVIER IRELAND LTD |
| 文献子类 | Article |
| 出版地 | CLARE |
| EISSN | 1873-2259 |
| 资助机构 | Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui Province [1708085QC51] ; Collegiate Natural Science Foundation of Anhui Province [KJ2016A223] ; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470689, 31570694, 31300577] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [BS2015NY003] ; Biology Key Subject Construction of Anhui |
| 作者邮箱 | xiatao62@126.com |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/20628 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Hefei, Anhui, Peoples R China 2.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Qingdao Agr Univ, Coll Hort, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Xiaolan,Huang, Keyi,Zheng, Guangshun,et al. CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis[J]. PLANT SCIENCE,2018,270:209-220. |
| APA | Jiang, Xiaolan.,Huang, Keyi.,Zheng, Guangshun.,Hou, Hua.,Wang, Peiqiang.,...&Xia, Tao.(2018).CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis.PLANT SCIENCE,270,209-220. |
| MLA | Jiang, Xiaolan,et al."CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis".PLANT SCIENCE 270(2018):209-220. |
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