IB-CAS  > 中科院北方资源植物重点实验室
The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides
Sun, Xiaoming1; Tomas Matus, Jose2; Wong, Darren Chern Jan3; Wang, Zemin; Chai, Fengmei1; Zhang, Langlang1; Fang, Ting1; Zhao, Li1; Wang, Yi; Han, Yuepeng1; Wang, Qingfeng1; Li, Shaohua; Liang, Zhenchang; Xin, Haiping1
2018
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
Volume69Issue:7Pages:1749-1764
AbstractGrapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera, and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and in Amur grape calli. In Arabidopsis, the ectopic expression of VaAQ increased antioxidant enzyme activities and up-regulated reactive oxygen species-(ROS) scavenging-related genes. Comparative mRNA sequencing profiling of 35S: VaAQ Arabidopsis plants suggests that this transcription factor is related to phosphate homeostasis like their Arabidopsis closest homologues: AtHRS1 and AtHHO2. However, when a cold stress is imposed, AQ is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GoLS) and raffinose synthase genes. Gene co-expression network (GCN) and cis-regulatory element (CRE) analyses in grapevine indicated AQ as potentially regulating VvGoLS genes. Increased RFO content was confirmed in both transgenic Arabidopsis and Amur grape calli overexpressing VaAQ. Taken together, our results imply that AQ improves cold tolerance through promoting the accumulation of osmoprotectants.
KeywordAbiotic stress Chinese wild grape cold response galactinol synthase grape transformation HRS1 low temperature exotherms raffinose ROS
Subject AreaPlant Sciences
DOI10.1093/jxb/ery020
Indexed BySCI
Language英语
WOS KeywordGALACTINOL SYNTHASE ; FREEZING TOLERANCE ; CHILLING STRESS ; GENE ; ACCLIMATION ; ANTIOXIDANTS ; DAMAGE ; OVEREXPRESSION ; EXPRESSION ; PROTECTION
WOS IDWOS:000428655800023
PublisherOXFORD UNIV PRESS
SubtypeArticle
Publication PlaceOXFORD
EISSN1460-2431
Funding OrganizationYouth Innovation Promotion Association of CAS [2015281] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M601166] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
Corresponding Author Emailzl249@ibcas.ac.cn ; xinhaiping215@hotmail.com
OAGreen Published, hybrid
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Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/20649
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China
3.CSIC IRTA UAB UB, CRAG, Barcelona 08193, Spain
4.Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
Recommended Citation
GB/T 7714
Sun, Xiaoming,Tomas Matus, Jose,Wong, Darren Chern Jan,et al. The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(7):1749-1764.
APA Sun, Xiaoming.,Tomas Matus, Jose.,Wong, Darren Chern Jan.,Wang, Zemin.,Chai, Fengmei.,...&Xin, Haiping.(2018).The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides.JOURNAL OF EXPERIMENTAL BOTANY,69(7),1749-1764.
MLA Sun, Xiaoming,et al."The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides".JOURNAL OF EXPERIMENTAL BOTANY 69.7(2018):1749-1764.
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