The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides

doi:10.1093/jxb/ery020

	The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides
	Sun, Xiaoming 1; Tomas Matus, Jose 2; Wong, Darren Chern Jan 3; Wang, Zemin ; Chai, Fengmei 1; Zhang, Langlang 1; Fang, Ting 1; Zhao, Li 1; Wang, Yi ; Han, Yuepeng 1; Wang, Qingfeng 1; Li, Shaohua; Liang, Zhenchang ; Xin, Haiping 1
	2018
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	69 期号:7 页码:1749-1764
摘要	Grapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera, and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and in Amur grape calli. In Arabidopsis, the ectopic expression of VaAQ increased antioxidant enzyme activities and up-regulated reactive oxygen species-(ROS) scavenging-related genes. Comparative mRNA sequencing profiling of 35S: VaAQ Arabidopsis plants suggests that this transcription factor is related to phosphate homeostasis like their Arabidopsis closest homologues: AtHRS1 and AtHHO2. However, when a cold stress is imposed, AQ is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GoLS) and raffinose synthase genes. Gene co-expression network (GCN) and cis-regulatory element (CRE) analyses in grapevine indicated AQ as potentially regulating VvGoLS genes. Increased RFO content was confirmed in both transgenic Arabidopsis and Amur grape calli overexpressing VaAQ. Taken together, our results imply that AQ improves cold tolerance through promoting the accumulation of osmoprotectants.
关键词	Abiotic stress Chinese wild grape cold response galactinol synthase grape transformation HRS1 low temperature exotherms raffinose ROS
学科领域	Plant Sciences
DOI	10.1093/jxb/ery020
收录类别	SCI
语种	英语
WOS关键词	GALACTINOL SYNTHASE ; FREEZING TOLERANCE ; CHILLING STRESS ; GENE ; ACCLIMATION ; ANTIOXIDANTS ; DAMAGE ; OVEREXPRESSION ; EXPRESSION ; PROTECTION
WOS记录号	WOS:000428655800023
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Youth Innovation Promotion Association of CAS [2015281] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M601166] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
作者邮箱	zl249@ibcas.ac.cn ; xinhaiping215@hotmail.com
作品OA属性	Green Published, hybrid
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20649
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 3.CSIC IRTA UAB UB, CRAG, Barcelona 08193, Spain 4.Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
推荐引用方式 GB/T 7714	Sun, Xiaoming,Tomas Matus, Jose,Wong, Darren Chern Jan,et al. The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(7):1749-1764.
APA	Sun, Xiaoming.,Tomas Matus, Jose.,Wong, Darren Chern Jan.,Wang, Zemin.,Chai, Fengmei.,...&Xin, Haiping.(2018).The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides.JOURNAL OF EXPERIMENTAL BOTANY,69(7),1749-1764.
MLA	Sun, Xiaoming,et al."The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides".JOURNAL OF EXPERIMENTAL BOTANY 69.7(2018):1749-1764.

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