Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control

doi:10.1111/tpj.13939

	Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control
	Zhang, Qian-Qian ; Li, Ying ; Fu, Zhao-Ying ; Liu, Xun-Biao ; Yuan, Kai ; Fang, Ying ; Liu, Yan 1; Li, Gang ; Zhang, Xian-Sheng ; Chong, Kang 1; Ge, Lei
	2018
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	95 期号:1 页码:150-167
摘要	Plant meristem activity depends on accurate execution of transcriptional networks required for establishing optimum functioning of stem cell niches. An Arabidopsis mutant card1-1 (constitutive auxin response with DR5: GFP) that encodes a truncated RPB1 (RNA Polymerase II's largest subunit) with shortened C-terminal domain (CTD) was identified. Phosphorylation of the CTD repeats of RPB1 is coupled to transcription in eukaryotes. Here we uncover that the truncated CTD of RPB1 disturbed cell cycling and enlarged the size of shoot and root meristem. The defects in patterning of root stem cell niche in card1-1 indicates that intact CTD of RPB1 is necessary for fine-tuning the specific expression of genes responsible for cell-fate determination. The gene-edited plants with different CTD length of RPB1, created by CRISPR-CAS9 technology, confirmed that both the full length and the DK-rich tail of RPB1' s CTD play roles in the accurate transcription of CYCB1; 1 encoding a cell-cycle marker protein in root meristem and hence participate in maintaining root meristem size. Our experiment proves that the intact RPB1 CTD is necessary for stem cell niche maintenance, which is mediated by transcriptional regulation of cell cycling genes.
关键词	Arabidopsis cell cycling RPB1 stem cell niche
学科领域	Plant Sciences
DOI	10.1111/tpj.13939
收录类别	SCI
语种	英语
WOS关键词	RNA-POLYMERASE-II ; ROOT-MERISTEM SIZE ; C-TERMINAL DOMAIN ; GENE-EXPRESSION ; CTD ; AUXIN ; TRANSCRIPTION ; SHOOT ; GROWTH ; PLANT
WOS记录号	WOS:000435446700013
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071066, 31370325] ; Taishan Scholar Program of Shandong Province
作者邮箱	gelei@sdau.edu.cn
作品OA属性	Bronze
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20665
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qian-Qian,Li, Ying,Fu, Zhao-Ying,et al. Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control[J]. PLANT JOURNAL,2018,95(1):150-167.
APA	Zhang, Qian-Qian.,Li, Ying.,Fu, Zhao-Ying.,Liu, Xun-Biao.,Yuan, Kai.,...&Ge, Lei.(2018).Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control.PLANT JOURNAL,95(1),150-167.
MLA	Zhang, Qian-Qian,et al."Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control".PLANT JOURNAL 95.1(2018):150-167.

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