The mode of action of remorin1 in regulating fruit ripening at transcriptional and post-transcriptional levels

doi:10.1111/nph.15264

	The mode of action of remorin1 in regulating fruit ripening at transcriptional and post-transcriptional levels
	Cai, Jianghua 1; Qin, Guozheng2 ; Chen, Tong 2; Tian, Shiping1,2
	2018
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	219 期号:4 页码:1406-1420
摘要	Remorins are plant-specific and plasma membrane-associated proteins that display a variety of functions in plant growth, development, biotic and abiotic stresses, and signal transduction. However, little information is available for understanding their role in fruit ripening. Here, remorin 1 (SlREM1) is cloned from tomato and its localization is examined by co-localization analysis and immunoblotting. Functions of SlREM1 in fruit ripening are characterized based on gene expression, co-immunoprecipitation coupled with mass spectroscopy and split luciferase complementation imaging assays in SlREM1 overexpression and RNA interference (RNAi) lines. The results indicate that SlREM1 is localized at the plasma membrane. Overexpression of SlREM1 in tomato stimulates fruit ripening with an increase in ethylene production and lycopene accumulation as compared to the wild-type. Consistently, these genes involved in ethylene and lycopene biosynthesis and ripening regulators also are upregulated in SlREM1 overexpression lines. SlREM1 can interact with ethylene biosynthesis proteins SAM1, ACO1 and ACS2 and is degraded by ubiquitin-mediated proteolysis. Our findings reveal that SlREM1 serves as a positive regulator of fruit ripening and provide novel cues for understanding of the molecular regulation network of fruit ripening.
关键词	ethylene biosynthesis fruit ripening lycopene accumulation protein interaction Remorin
学科领域	Plant Sciences
DOI	10.1111/nph.15264
收录类别	SCI
语种	英语
WOS关键词	TOMATO FRUIT ; 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE ; DIFFERENTIAL EXPRESSION ; PROTEOMICS REVEALS ; ETHYLENE CONTROL ; ABSCISIC-ACID ; PROTEIN ; GENE ; BIOSYNTHESIS ; MEMBRANE
WOS记录号	WOS:000440847600026
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1469-8137
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31672210]
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20672
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Agr, Key Lab Post Harvest Handing Fruits, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Cai, Jianghua,Qin, Guozheng,Chen, Tong,et al. The mode of action of remorin1 in regulating fruit ripening at transcriptional and post-transcriptional levels[J]. NEW PHYTOLOGIST,2018,219(4):1406-1420.
APA	Cai, Jianghua,Qin, Guozheng,Chen, Tong,&Tian, Shiping.(2018).The mode of action of remorin1 in regulating fruit ripening at transcriptional and post-transcriptional levels.NEW PHYTOLOGIST,219(4),1406-1420.
MLA	Cai, Jianghua,et al."The mode of action of remorin1 in regulating fruit ripening at transcriptional and post-transcriptional levels".NEW PHYTOLOGIST 219.4(2018):1406-1420.

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