In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy

doi:10.1016/j.jplph.2017.11.002

	In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy
	Man, Yi ; Zhao, Yuanyuan ; Ye, Rong ; Lin, Jinxing 1; Jing, Yanping
	2018
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	220 页码:136-144
摘要	The Casparian strip, a barrier to the apoplastic movement of solutes from the cortex to the stele, is essential for the exclusion of salts, selective nutrient uptake, and many other processes. To date, extensive studies have focused on the physiological functions of endodermal Casparian strips. However, the chemical deposition nature of Casparian strips, as well as its relevance with respect to diffusion barrier functions, remains to be further elucidated. Here, we revealed three developmental stages of Casparian strips in maize primary roots using a traditional fluorescent staining method. Apoplastic permeability tests demonstrated that the barrier function of Casparian strips is largely related to their developmental stage and the pattern of lignin and suberin deposits. Fourier transform infrared (FTIR) analysis showed that the Casparian strips from the roots exhibited significant absorption bands characteristic of lignin and suberin, implying that the Casparian strips in maize primary roots consist largely of lignin and suberin. Furthermore, we developed a new method for label-free, in vivo structural, and biochemical analysis of Casparian strips based on stimulated Raman scattering (SRS) microscopy. Using SRS microscopy, we found that lignin and suberin accumulate simultaneously during the Casparian strip formation process. Based on these results, we propose a potential application of SRS for the chemical composition analysis of plant Casparian strips in situ.
关键词	Casparian strip Lignin Suberin Label-free analysis Stimulated Raman scattering microscopy Maize
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2017.11.002
收录类别	SCI
语种	英语
WOS关键词	LABEL-FREE ; FINE-STRUCTURE ; ROOTS ; LIGNIN ; GROWTH ; TRANSPORT ; BARRIERS ; SUBERIN ; MAIZE ; SALT
WOS记录号	WOS:000422925800014
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	National Key Research and Development Program of China [2017YFD0600201] ; Program of Introducing Talents of Discipline to Universities (111 project) [B13007]
作者邮箱	ypjing@bjfu.edu.cn
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20679
专题	中科院北方资源植物重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Box 162,35 Qinghua East Rd, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Man, Yi,Zhao, Yuanyuan,Ye, Rong,et al. In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy[J]. JOURNAL OF PLANT PHYSIOLOGY,2018,220:136-144.
APA	Man, Yi,Zhao, Yuanyuan,Ye, Rong,Lin, Jinxing,&Jing, Yanping.(2018).In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy.JOURNAL OF PLANT PHYSIOLOGY,220,136-144.
MLA	Man, Yi,et al."In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy".JOURNAL OF PLANT PHYSIOLOGY 220(2018):136-144.

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