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Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus
Liu, Jinyue1,2,3; Jiang, Wenbo1; Xia, Yaying1; Wang, Xuemin3; Shen, Guoan4; Pang, Yongzhen1,3
2018
发表期刊PLANT AND CELL PHYSIOLOGY
ISSN0032-0781
卷号59期号:1页码:128-141
摘要Prenylated isoflavonoids have been found in several legume plants, and they possess various biological activities that play important roles in both plant defense and human health. However, it is still unknown whether prenylated isoflavonoids are present in the model legume plant Lotus japonicus. In the present study, we found that the prenylated isoflavonoid wighteone was produced in L. japonicus when leaf was supplemented with genistein. Furthermore, a novel prenyltransferase gene, LjG6DT, was identified, which shared high similarity with and was closely related to several known prenyltransferase genes involved in isoflavonoid biosynthesis. The recombinant LjG6DT protein expressed in yeast exhibited prenylation activity toward genistein as an exclusive substrate, which produced wighteone, a prenylated genistein at the C-6 position that occurs normally in legume plants. The LjG6DT-green fluorescent protein (GFP) fusion protein is targeted to plastids. The transcript level of LjG6DT is induced by glutathione, methyl jasmonate and salicylic acid, implying that LjG6DT is involved in stress response. Overexpression of LjG6DT in L. japonicus hairy roots led to increased accumulation of wighteone when genistein was supplied, indicating that LjG6DT is functional in vivo. Feeding assays with the upstream intermediate naringenin revealed that accumulation of wighteone in L. japonicus was dependent on genistein supplementation, and accumulation of wighteone is competed by genistein methylation. This study demonstrated that phytoalexin wighteone is inducibly produced in L. japonicus, and it provides new insight into the biosynthesis and accumulation of prenylated isoflavonoids in legume plants.
关键词Genistein Isoflavonoids Isoflavonoid prenyl-transferase Lotus japonicus Wighteone
学科领域Plant Sciences ; Cell Biology
DOI10.1093/pcp/pcx167
收录类别SCI
语种英语
WOS关键词MOLECULAR CHARACTERIZATION ; ISOFLAVONOID BIOSYNTHESIS ; PRENYLATED ISOFLAVONOIDS ; ENZYMATIC PRENYLATION ; PRENYLTRANSFERASE ; FLAVONOIDS ; IDENTIFICATION ; PROTEIN ; CLONING ; GLYCEOLLIN
WOS记录号WOS:000423660600019
出版者OXFORD UNIV PRESS
文献子类Article
出版地OXFORD
EISSN1471-9053
资助机构International S&T Cooperation Program of China [2015DFR30570-3] ; Major State Basic Research and Development ProgramNational Basic Research Program of China [2014CB138703] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305]
作者邮箱shenguoan@ibcas.ac.cn ; yzhpang@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/20689
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
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Liu, Jinyue,Jiang, Wenbo,Xia, Yaying,et al. Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus[J]. PLANT AND CELL PHYSIOLOGY,2018,59(1):128-141.
APA Liu, Jinyue,Jiang, Wenbo,Xia, Yaying,Wang, Xuemin,Shen, Guoan,&Pang, Yongzhen.(2018).Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus.PLANT AND CELL PHYSIOLOGY,59(1),128-141.
MLA Liu, Jinyue,et al."Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus".PLANT AND CELL PHYSIOLOGY 59.1(2018):128-141.
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