DNA barcoding of invasive plants in China: A resource for identifying invasive plants

doi:10.1111/1755-0998.12715

	DNA barcoding of invasive plants in China: A resource for identifying invasive plants
	Xu, Song-Zhi ; Li, Zhen-Yu ; Jin, Xiao-Hua
	2018
发表期刊	MOLECULAR ECOLOGY RESOURCES
ISSN	1755-098X
卷号	18 期号:1 页码:128-136
摘要	Invasive plants have aroused attention globally for causing ecological damage and having a negative impact on the economy and human health. However, it can be extremely challenging to rapidly and accurately identify invasive plants based on morphology because they are an assemblage of many different families and many plant materials lack sufficient diagnostic characteristics during border inspections. It is therefore urgent to evaluate candidate loci and build a reliable genetic library to prevent invasive plants from entering China. In this study, five common single markers (ITS, ITS2, matK, rbcL and trnH-psbA) were evaluated using 634 species (including 469 invasive plant species in China, 10 new records to China, 16 potentially invasive plant species around the world but not introduced into China yet and 139 plant species native to China) based on three different methods. Our results indicated that ITS2 displayed largest intra- and interspecific divergence (1.72% and 91.46%). Based on NJ tree method, ITS2, ITS, matK, rbcL and trnH-psbA provided 76.84%, 76.5%, 63.21%, 52.86% and 50.68% discrimination rates, respectively. The combination of ITS + matK performed best and provided 91.03% discriminatory power, followed by ITS2 + matK (85.78%). For identifying unknown individuals, ITS + matK had 100% correct identification rate based on our database, followed by ITS/ITS2 (both 93.33%) and ITS2 + matK (91.67%). Thus, we propose ITS/ITS2 + matK as the most suitable barcode for invasive plants in China. This study also demonstrated that DNA barcoding is an efficient tool for identifying invasive species.
关键词	DNA barcoding invasive plant species ITS/ITS2+matK molecular marker
学科领域	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI	10.1111/1755-0998.12715
收录类别	SCI
语种	英语
WOS关键词	ALIEN PLANT ; PHYLOGENY ; IDENTIFICATIONS ; DISCRIMINATE ; PISCES ; SPACER
WOS记录号	WOS:000424112000012
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1755-0998
资助机构	STS Program of Chinese Academy of Sciences [ZSSD-018]
作者邮箱	lizy@ibcas.ac.cn ; xiaohuajin@ibcas.ac.cn
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20690
专题	系统与进化植物学国家重点实验室
作者单位	1.[Xu, Song-Zhi 2.Li, Zhen-Yu 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Song-Zhi,Li, Zhen-Yu,Jin, Xiao-Hua. DNA barcoding of invasive plants in China: A resource for identifying invasive plants[J]. MOLECULAR ECOLOGY RESOURCES,2018,18(1):128-136.
APA	Xu, Song-Zhi,Li, Zhen-Yu,&Jin, Xiao-Hua.(2018).DNA barcoding of invasive plants in China: A resource for identifying invasive plants.MOLECULAR ECOLOGY RESOURCES,18(1),128-136.
MLA	Xu, Song-Zhi,et al."DNA barcoding of invasive plants in China: A resource for identifying invasive plants".MOLECULAR ECOLOGY RESOURCES 18.1(2018):128-136.

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