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| QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population | |
Qin Yaobin; Cheng Peng1; Cheng Yichen; Feng Yue; Huang Derun; Huang Tingxu2; Song Xianjun1 ; Ying Jiezheng
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| 2018 | |
| Source Publication | RICE SCIENCE
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| ISSN | 1672-6308 |
| Volume | 25Issue:3Pages:121-131 |
| Abstract | Mapping and isolation of quantitative trait loci (QTLs) or genes controlling grain size or weight is very important to uncover the molecular mechanisms of seed development and crop breeding. To identify the QTLs controlling grain size and weight, we developed a near isogenic line F-2 (NIL-F-2) population, which was derived from a residual heterozygous plant in an F-7 generation of recombinant inbred line (RIL). With the completion of more than 30x whole genome re-sequencing of the parents, two DNA bulks for large and small grains, a total of 58.94 Gb clean nucleotide data were generated. A total of 455 262 single nucleotide polymorphisms (SNPs) between the parents were identified to perform bulked QTL-seq. A candidate genomic region containing SNPs strongly associated with grain length and weight was identified from 15 to 20 Mb on chromosome 5. We designated the major QTL in the candidate region as qTGW5.3. Then, qTGW5.3 was further validated with PCR-based conventional QTL mapping method through developing simple sequence repeat and Insertion/Deletion markers in the F-2 population. Furthermore, recombinants and the progeny tests delimited the candidate region of qTGW5.3 to 1.13 Mb, flanked by HX5009 (15.15 Mb) and HX5003 (16.28 Mb). A set of NILs, selected from the F-2 population, was developed to evaluate the genetic effect of qTGW5.3. Significant QTL effects were detected on grain length, grain width and 1000-grain weight of H12-29 allele with 1.14 mm, -0.11 mm and 3.11 g, which explained 99.64%, 95.51% and 97.32% of the phenotypic variations, respectively. |
| Keyword | grain length grain weight QTL-seq quantitative trait locus near-isogenic line rice single nucleotide polymorphism recombinant inbred line qTGW5.3 |
| Subject Area | Agronomy ; Plant Sciences |
| DOI | 10.1016/j.rsci.2018.04.001 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | QUANTITATIVE TRAIT LOCI ; SIZE DIVERSITY ; MARKERS ; YIELD ; CONTRIBUTES ; DISSECTION ; PROGRESS ; ALLELE ; POOLS ; SHAPE |
| WOS ID | WOS:000432465300001 |
| Publisher | ELSEVIER |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 1876-4762 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371605] ; Chinese High-Yielding Transgenic Program [2016ZX08001-004] |
| Corresponding Author Email | songxj@ibcas.ac.cn ; yingjiezheng@caas.cn |
| OA | gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/20700 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Fujian Acad Agr Sci, Fuzhou Natl Subctr Rice Improvement, Inst Rice Res, Fuzhou 350018, Fujian, Peoples R China |
| Recommended Citation GB/T 7714 | Qin Yaobin,Cheng Peng,Cheng Yichen,et al. QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population[J]. RICE SCIENCE,2018,25(3):121-131. |
| APA | Qin Yaobin.,Cheng Peng.,Cheng Yichen.,Feng Yue.,Huang Derun.,...&Ying Jiezheng.(2018).QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population.RICE SCIENCE,25(3),121-131. |
| MLA | Qin Yaobin,et al."QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population".RICE SCIENCE 25.3(2018):121-131. |
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| Applet_2022-1-13_164（1999KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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