Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes

doi:10.1007/s00299-018-2302-9

	Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes
	Zhang, Langlang 2; Cheng, Jun 1; Sun, Xiaoming 1; Zhao, Tingting ; Li, Mingjun 3; Wang, Qingfeng ; Li, Shaohua1 ; Xin, Haiping
	2018
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	37 期号:8 页码:1159-1172
摘要	Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes, including COR15A, COR15B, COR413, KIN2, and RD29A. The WRKY family is one of a largest transcription factors in plants, and it is a key component of multiple stress responses. In this study, the drought- and cold-induced WRKY family gene VaWRKY14 was isolated and characterized. Phylogenetic analysis indicated that VaWRKY14 belongs to the WRKY IIa subfamily, of which several members participate in biotic and abiotic stress responses in plants. Fluorescence observation from Arabidopsis mesophyll protoplasts transformed with the VaWRKY14::eGFP fusion vector suggested that VaWRKY14 was localized in the nucleus. The VaWRKY14 in yeast cells did not display any transcriptional activity. The expression of VaWRKY14 could be induced by exogenous phytohormones, including salicylic acid (SA) and abscisic acid (ABA). Overexpression of VaWRKY14 enhanced the drought tolerance of transgenic Arabidopsis. Compared with wild-type Arabidopsis, the VaWRKY14-OE lines exhibited higher water content and antioxidant enzyme activities in leaves after drought treatment. RNA sequencing analysis revealed that several stress-related genes, including COR15A, COR15B, COR413, KIN2, and RD29A, were upregulated in transgenic plants relative to their expression in wild-type Arabidopsis under normal conditions. Several genes (3 upregulated and 49 down-regulated) modulated by VaWRKY14 were also affected by drought stress in wild-type plants. These data suggest that VaWRKY14 responds to drought and cold stresses and that drought tolerance may be enhanced by regulating the expression of stress-related genes in Arabidopsis.
关键词	VaWRKY14 WRKY IIa Transcriptome Drought Grapevine
学科领域	Plant Sciences
DOI	10.1007/s00299-018-2302-9
收录类别	SCI
语种	英语
WOS关键词	WRKY TRANSCRIPTION FACTORS ; ABSCISIC-ACID ; COLD STRESS ; FORTUNELLA-CRASSIFOLIA ; NEGATIVE REGULATORS ; DISEASE RESISTANCE ; SALICYLIC-ACID ; DNA-BINDING ; RNA-SEQ ; RICE
WOS记录号	WOS:000438935500007
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-203X
资助机构	National Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31471857] ; Youth Innovation Promotion Association of CAS [2015281] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502] ; Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas (NWAFU) [CSBAA2016009]
作者邮箱	xinhaiping215@hotmail.com
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20710
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Langlang,Cheng, Jun,Sun, Xiaoming,et al. Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes[J]. PLANT CELL REPORTS,2018,37(8):1159-1172.
APA	Zhang, Langlang.,Cheng, Jun.,Sun, Xiaoming.,Zhao, Tingting.,Li, Mingjun.,...&Xin, Haiping.(2018).Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes.PLANT CELL REPORTS,37(8),1159-1172.
MLA	Zhang, Langlang,et al."Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes".PLANT CELL REPORTS 37.8(2018):1159-1172.

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