Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains

doi:10.1073/pnas.1806304115

	Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains
	Liu, Jinxin ; Wu, Xiaoba ; Yao, Xuefeng ; Yu, Ronald ; Larkin, Philip J.; Liu, Chun-Ming
	2018
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	115 期号:44 页码:11327-11332
摘要	The rice endosperm, consisting of an outer single-cell layer aleurone and an inner starchy endosperm, is an important staple food for humans. While starchy endosperm stores mainly starch, the aleurone is rich in an array of proteins, vitamins, and minerals. To improve the nutritional value of rice, we screened for mutants with thickened aleurones using a half-seed assay and identified thick aleurone 2-1 (tat-1), in which the aleurone has 4.8 +/- 2.2 cell layers on average. Except for starch, the contents of all measured nutritional factors, including lipids, proteins, vitamins, minerals, and dietary fibers, were increased in tat-1 grains. Map-based cloning showed that TA2 encodes the DNA demethylase OsROS1. A point mutation in the 14th intron of OsROS1 led to alternative splicing that generated an extra transcript, mOsROS1, with a 21-nt insertion from the intron. Genetic analyses showed that the tat-1 phenotype is inherited with an unusual gametophytic maternal effect, which is caused not by imprinted gene expression but rather by the presence of the mOsROS1 transcript. Five additional tat alleles with the increased aleurone cell layer and different inheritance patterns were identified by TILLING. Genome-wide bisulfite sequencing revealed general increases in CG and CHG methylations in tat-1 endosperms, along with hypermethylation and reduced expression in two putative aleurone differentiation-related transcription factors. This study thus suggests that OsROS1-mediated DNA demethylation restricts the number of aleurone cell layers in rice and provides a way to improve the nutrition of rice.
关键词	rice endosperm thick aleurone nutrition DNA demethylation
学科领域	Multidisciplinary Sciences
DOI	10.1073/pnas.1806304115
收录类别	SCI
语种	英语
WOS关键词	MAIZE ENDOSPERM ; CELL-LAYERS ; GENE ; ARABIDOPSIS ; DIFFERENTIATION ; ENCODES ; PROTEIN ; FORTIFICATION ; CRINKLY4 ; DEMETER
WOS记录号	WOS:000448713200065
出版者	NATL ACAD SCIENCES
文献子类	Article
出版地	WASHINGTON
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0100501/2014CB943401] ; Chinese Academy of Sciences (CAS) Innovation Project Molecular Modules for Breeding Design [XDA08010301] ; CAS-Commonwealth Scientific and Industrial Research Organization Bilateral Collaboration Project [GJHZ1406]
作者邮箱	cmliu@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：89[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20713
专题	中科院植物分子生理学重点实验室
作者单位	1.[Liu, Jinxin 2.Wu, Xiaoba 3.Yao, Xuefeng 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.[Yu, Ronald 6.Larkin, Philip J.] CSIRO, Agr & Food, Canberra, ACT 2601, Australia 7.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jinxin,Wu, Xiaoba,Yao, Xuefeng,et al. Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(44):11327-11332.
APA	Liu, Jinxin,Wu, Xiaoba,Yao, Xuefeng,Yu, Ronald,Larkin, Philip J.,&Liu, Chun-Ming.(2018).Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(44),11327-11332.
MLA	Liu, Jinxin,et al."Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.44(2018):11327-11332.

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