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Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass
Yu, Juanjuan1; Zhang, Yongxue1; Liu, Junming; Wang, Lin; Liu, Panpan; Yin, Zepeng; Guo, Siyi2; Ma, Jun3; Lu, Zhuang4; Wang, Tai4; She, Yimin3; Miao, Yuchen2; Ma, Ling; Chen, Sixue5; Li, Ying; Dai, Shaojun1
2018
发表期刊PLANTA
ISSN0032-0935
卷号248期号:5页码:1079-1099
摘要Main conclusion Hydrogen peroxide-responsive pathways in roots of alkaligrass analyzed by proteomic studies and PutGLP enhance the plant tolerance to saline-, alkali- and cadmium-induced oxidative stresses. Oxidative stress adaptation is critical for plants in response to various stress environments. The halophyte alkaligrass (Puccinellia tenuiflora) is an outstanding pasture with strong tolerance to salt and alkali stresses. In this study, iTRAQ- and 2DE-based proteomics approaches, as well as qRT-PCR and molecular genetics, were employed to investigate H2O2-responsive mechanisms in alkaligrass roots. The evaluation of membrane integrity and reactive oxygen species (ROS)-scavenging systems, as well as abundance patterns of H2O2-responsive proteins/genes indicated that Ca2+-mediated kinase signaling pathways, ROS homeostasis, osmotic modulation, and transcriptional regulation were pivotal for oxidative adaptation in alkaligrass roots. Overexpressing a P. tenuiflora germin-like protein (PutGLP) gene in Arabidopsis seedlings revealed that the apoplastic PutGLP with activities of oxalate oxidase and superoxide dismutase was predominantly expressed in roots and played important roles in ROS scavenging in response to salinity-, alkali-, and CdCl2-induced oxidative stresses. The results provide insights into the fine-tuned redox-responsive networks in halophyte roots.
关键词Germin-like protein Hydrogen peroxide response Proteomics Puccinellia tenuiflora Roots Signaling
学科领域Plant Sciences
DOI10.1007/s00425-018-2940-8
收录类别SCI
语种英语
WOS关键词HYDROGEN-PEROXIDE STRESS ; PUCCINELLIA-TENUIFLORA ; OXIDATIVE STRESS ; PLASMA-MEMBRANE ; SALT STRESS ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS ; ANALYSIS REVEALS ; NITRIC-OXIDE ; CROSS-TALK
WOS记录号WOS:000447030900004
出版者SPRINGER
文献子类Article
出版地NEW YORK
EISSN1432-2048
资助机构National Key Research and Development Program [2017YFD0600101] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2572017ET01, 2572017EA05, 2572016AA16] ; Foundation of Shanghai Science and Technology Committee, China [17391900600]
作者邮箱ly7966@nefu.edu.cn ; daishaojun@hotmail.com
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/20723
专题中科院植物分子生理学重点实验室
作者单位1.Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat Minis, Alkali Soil Nat Environm Sci Ctr, Harbin 150040, Heilongjiang, Peoples R China
2.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China
3.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 455000, Peoples R China
4.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China
5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
6.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Plant Mol & Cellular Program, Genet Inst,Dept Biol, Gainesville, FL 32610 USA
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Yu, Juanjuan,Zhang, Yongxue,Liu, Junming,et al. Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass[J]. PLANTA,2018,248(5):1079-1099.
APA Yu, Juanjuan.,Zhang, Yongxue.,Liu, Junming.,Wang, Lin.,Liu, Panpan.,...&Dai, Shaojun.(2018).Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass.PLANTA,248(5),1079-1099.
MLA Yu, Juanjuan,et al."Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass".PLANTA 248.5(2018):1079-1099.
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