RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.)

doi:10.1016/j.bbrc.2019.11.084

	RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.)
	Wu, Ming-Wei ; Zhao, Heng ; Zhang, Jin-Dan ; Guo, Lei 3; Liu, Chun-Ming
	2020
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	523 期号:1 页码:220-225
摘要	Pentatricopeptide repeat (PPR) proteins are one of the largest protein families in land plants. PPR proteins exhibit sequence-specific RNA-binding activity and are implicated in plant growth and development related processes. In this study, we report that the radicleless 1 (rl1) mutant in rice (Oryza sativa L.) exhibited defective radicle emergence in embryos and compromised grain filling in endosperms. Gene cloning and confirmation via genetic complementation analyses showed that RL1 encodes a P-type PPR protein, which is localized to mitochondria. The RL1 protein was specifically involved in the splicing of intron 1 of the mitochondrial nad4 transcript, which encodes a subunit of the mitochondrial NADH dehydrogenase complex. Consistent with this observation, the rl1 mutant exhibited altered mitochondrial morphology and lower ATP accumulation compared with the wild type. Thus, our findings suggest that RL1-mediated nad4 splicing is crucial for embryo and endosperm development in rice. (C) 2019 Elsevier Inc. All rights reserved.
关键词	Pentatricopeptide repeat RL1 splicing nad4 Mitochondrial morphology
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbrc.2019.11.084
收录类别	SCI
语种	英语
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEINS ; SEED DEVELOPMENT ; PPR PROTEIN ; EXPRESSION ; ENCODES
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000524719500036
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
文献子类	Article
出版地	SAN DIEGO
EISSN	1090-2104
资助机构	Beijing Municipal Science and Technology Commission of China [Z181100002418010] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0100501/2014CB943401] ; Chinese Academy of Sciences (CAS) Innovation Project Molecular Modules for Breeding Design [XDA08010301]
作者邮箱	cmliu@ibcas.ac.cn
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21457
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 4.Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA 5.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ming-Wei,Zhao, Heng,Zhang, Jin-Dan,et al. RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,523(1):220-225.
APA	Wu, Ming-Wei,Zhao, Heng,Zhang, Jin-Dan,Guo, Lei,&Liu, Chun-Ming.(2020).RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,523(1),220-225.
MLA	Wu, Ming-Wei,et al."RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 523.1(2020):220-225.

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