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| Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics | |
Suo, Jinwei1,2; Zhang, Heng; Zhao, Qi1; Zhang, Nan; Zhang, Yongxue1; Li, Ying1; Song, Baohua1; Yu, Juanjuan1; Cao, Jianguo; Wang, Tai3 ; Luo, Ji4; Guo, Lihai4; Ma, Jun5; Zhang, Xumin6; She, Yimin5; Peng, Lianwei; Ma, Weimin; Guo, Siyi7; Miao, Yuchen7; Chen, Sixue8; Qin, Zhi; Dai, Shaojun1
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| 2020 | |
| Source Publication | GENOMICS PROTEOMICS & BIOINFORMATICS
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| ISSN | 1672-0229 |
| Volume | 18Issue:3Pages:271-288 |
| Abstract | Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuifiora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test the functions of the Na2CO3-responsive proteins. A total of 104 and 102 Na2CO3-responsive proteins were identified in leaves and chloroplasts, respectively. In addition, 84 Na2CO3-responsive phosphoproteins were identified, including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts, which are crucial for the regulation of photosynthesis, ion transport, signal transduction, and energy homeostasis. A full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, leading to enhanced Na2CO3 tolerance. All these results indicate that thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of photosystem (PS) II, PSI activity, and ROS homeostasis were altered in response to Na2CO3 stress, which help to improve our understanding of the Na2CO3-responsive mechanisms in halophytes. |
| Keyword | Chloroplasts Na2CO3 stress ROS scavenging Phosphoproteomics Puccinellia tenuiflora |
| Subject Area | Genetics & Heredity |
| DOI | 10.1016/j.gpb.2018.10.011 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | COMPARATIVE PROTEOMIC ANALYSIS ; PHOTOSYSTEM-II ; SALT-STRESS ; QUANTITATIVE PROTEOMICS ; NADPH DEHYDROGENASE ; SALINITY TOLERANCE ; ION BALANCE ; PROTEIN ; PHOSPHORYLATION ; LIGHT |
| WOS Research Area | Genetics & Heredity |
| WOS ID | WOS:000605184400005 |
| Publisher | ELSEVIER |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 2210-3244 |
| Funding Organization | Foundation of Shanghai Science and Technology CommitteeShanghai Science & Technology Committee [17391900600] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2011) ; Natural and Science Foundation of Heilongjiang Provence [ZD2019C003] ; Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2017) |
| Corresponding Author Email | qinzhi@shnu.edu.cn ; daishaojun@shnu.edu.cn |
| OA | Green Submitted, Green Published, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21481 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China 3.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China 6.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China 7.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China 8.Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China 9.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA |
| Recommended Citation GB/T 7714 | Suo, Jinwei,Zhang, Heng,Zhao, Qi,et al. Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2020,18(3):271-288. |
| APA | Suo, Jinwei.,Zhang, Heng.,Zhao, Qi.,Zhang, Nan.,Zhang, Yongxue.,...&Dai, Shaojun.(2020).Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics.GENOMICS PROTEOMICS & BIOINFORMATICS,18(3),271-288. |
| MLA | Suo, Jinwei,et al."Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics".GENOMICS PROTEOMICS & BIOINFORMATICS 18.3(2020):271-288. |
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| 1-s2.0-S167202292030（5164KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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