Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics

doi:10.1016/j.gpb.2018.10.011

	Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics
	Suo, Jinwei 1,2; Zhang, Heng ; Zhao, Qi 1; Zhang, Nan ; Zhang, Yongxue 1; Li, Ying 1; Song, Baohua 1; Yu, Juanjuan 1; Cao, Jianguo ; Wang, Tai3 ; Luo, Ji 4; Guo, Lihai 4; Ma, Jun 5; Zhang, Xumin 6; She, Yimin 5; Peng, Lianwei ; Ma, Weimin ; Guo, Siyi 7; Miao, Yuchen 7; Chen, Sixue 8; Qin, Zhi ; Dai, Shaojun 1
	2020
发表期刊	GENOMICS PROTEOMICS & BIOINFORMATICS
ISSN	1672-0229
卷号	18 期号:3 页码:271-288
摘要	Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuifiora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test the functions of the Na2CO3-responsive proteins. A total of 104 and 102 Na2CO3-responsive proteins were identified in leaves and chloroplasts, respectively. In addition, 84 Na2CO3-responsive phosphoproteins were identified, including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts, which are crucial for the regulation of photosynthesis, ion transport, signal transduction, and energy homeostasis. A full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, leading to enhanced Na2CO3 tolerance. All these results indicate that thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of photosystem (PS) II, PSI activity, and ROS homeostasis were altered in response to Na2CO3 stress, which help to improve our understanding of the Na2CO3-responsive mechanisms in halophytes.
关键词	Chloroplasts Na2CO3 stress ROS scavenging Phosphoproteomics Puccinellia tenuiflora
学科领域	Genetics & Heredity
DOI	10.1016/j.gpb.2018.10.011
收录类别	SCI
语种	英语
WOS关键词	COMPARATIVE PROTEOMIC ANALYSIS ; PHOTOSYSTEM-II ; SALT-STRESS ; QUANTITATIVE PROTEOMICS ; NADPH DEHYDROGENASE ; SALINITY TOLERANCE ; ION BALANCE ; PROTEIN ; PHOSPHORYLATION ; LIGHT
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000605184400005
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	2210-3244
资助机构	Foundation of Shanghai Science and Technology CommitteeShanghai Science & Technology Committee [17391900600] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2011) ; Natural and Science Foundation of Heilongjiang Provence [ZD2019C003] ; Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2017)
作者邮箱	qinzhi@shnu.edu.cn ; daishaojun@shnu.edu.cn
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21481
专题	中科院植物分子生理学重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China 3.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China 6.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China 7.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China 8.Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China 9.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA
推荐引用方式 GB/T 7714	Suo, Jinwei,Zhang, Heng,Zhao, Qi,et al. Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2020,18(3):271-288.
APA	Suo, Jinwei.,Zhang, Heng.,Zhao, Qi.,Zhang, Nan.,Zhang, Yongxue.,...&Dai, Shaojun.(2020).Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics.GENOMICS PROTEOMICS & BIOINFORMATICS,18(3),271-288.
MLA	Suo, Jinwei,et al."Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics".GENOMICS PROTEOMICS & BIOINFORMATICS 18.3(2020):271-288.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S167202292030（5164KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文