Knowledge Management System Of Institute Of Botany,CAS
Diatom DNA barcodes for forensic discrimination of drowning incidents | |
Liu, Mengyan1; Zhao, Yi1; Sun, Yuzhe2,3; Wu, Ping2,3; Zhou, Shiliang2,3; Ren, Liang | |
2020 | |
发表期刊 | FEMS MICROBIOLOGY LETTERS |
ISSN | 0378-1097 |
卷号 | 367期号:17 |
摘要 | The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S (V4 of 18S) and BacirbcL (central region of rbcL), for amplifying fragments of 16S/18S, 23S/28S, COI, ITS and rbcL. A total of five pairs of primers performed satisfactory for diatoms. We used three of them, 18S605 (V2 + V3 of 18S), Baci18S and BacirbcL, to barcode four water samples using next generation sequencing platform. The results showed that these primers worked well for NGS metabarcoding of diatoms. We suggest that 18S605, Baci18S and BacirbcL be barcodes of diatoms and the corresponding primer pairs be used. Considering a quite high proportion of sequences deposited in GenBank were mislabeled, the most urgent task for DNA barcoding of diatoms is to create standard sequences using correctly identified specimens, ideally type specimens. |
关键词 | 18S diatom DNA barcode primer rbcL |
学科领域 | Microbiology |
DOI | 10.1093/femsle/fnaa145 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | IDENTIFICATION ; SOFTWARE ; RBCL |
WOS研究方向 | Microbiology |
WOS记录号 | WOS:000602578400008 |
出版者 | OXFORD UNIV PRESS |
文献子类 | Article |
出版地 | OXFORD |
EISSN | 1574-6968 |
作者邮箱 | RENLIANG111223344@163.com |
作品OA属性 | Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21487 |
专题 | 系统与进化植物学国家重点实验室 |
作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, 13 HangkongRd, Wuhan 430030, Hubei, Peoples R China 2.Beijing Publ Secur Bur, Forens Judicial Appraisal Ctr, 1 Longgang Beijing Rd, Beijing 100192, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Xiangshan St, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Yuquan Rd, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,et al. Diatom DNA barcodes for forensic discrimination of drowning incidents[J]. FEMS MICROBIOLOGY LETTERS,2020,367(17). |
APA | Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,Wu, Ping,Zhou, Shiliang,&Ren, Liang.(2020).Diatom DNA barcodes for forensic discrimination of drowning incidents.FEMS MICROBIOLOGY LETTERS,367(17). |
MLA | Liu, Mengyan,et al."Diatom DNA barcodes for forensic discrimination of drowning incidents".FEMS MICROBIOLOGY LETTERS 367.17(2020). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Liu-2020-Diatom DNA (619KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论