Knowledge Management System Of Institute Of Botany,CAS
| Evaluation of Metal Tolerance of Fungal Strains Isolated from Contaminated Mining Soil of Nanjing, China | |
| Liaquat, Fiza; Munis, Muhammad Farooq Hussain1; Haroon, Urooj1; Arif, Samiah; Saqib, Saddam2,3; Zaman, Wajid2,3; Khan, Ali Raza4; Shi, Jianxin5; Che, Shengquan6; Liu, Qunlu6 | |
| 2020 | |
| 发表期刊 | BIOLOGY-BASEL
 |
| 卷号 | 9期号:12 |
| 摘要 | Simple Summary In this study, cadmium, chromium, and lead tolerant microbes have been isolated from contaminated mining soil and characterized. Molecular characterization of isolated fungi was performed and amplified sequences were deposited in the GenBank NCBI database. Metal tolerance of the various strains has been determined by measuring the minimum inhibitory concentrations (MICs) and the tolerance indexes of all the tested strains against Cd, Cr, and Pb. Bioaccumulation capacities of Trichoderma harzianum and Komagataella phaffi have also been assessed. These findings helped us find a novel strain of Komagataella phaffi and suggested it to be the potential mycoremediation microbe to alleviate the contamination of Cd, Cr, and Pb. Future studies of this fungal strain can help us to understand its resistance mechanism against other heavy metals, too. Rapidly increasing industry has resulted in greater discharge of hazardous chemicals in the soil. In the current study, soil samples were collected from Nanjing mine (32 degrees 09 ' 19.29 '' N 118 degrees 56 ' 57.04 '' E) and subjected to heavy metal analysis and microbe isolation. A total of 460 fungi were isolated, and five of these were yeast strains. Most of the strains exhibited tolerance to one metal. Five multimetal tolerant strains were selected and identified as Aspergillus sclerotiorum, Aspergillus aculeatus, Komagataella phaffii, Trichoderma harzianum, and Aspergillus niger. Isolated strains were grown in high concentrations of cadmium (Cd), chromium (Cr) and lead (Pb), for induced-tolerance training. The tolerance index (TI) revealed the highest Cd tolerance of novel K. phaffii strain at 5500 ppm (TI: 0.2). K. phaffii also displayed resistance at 4000 ppm against Cr (TI: 0.32) and Pb (TI: 0.32). In contrast, tolerance training for A. niger was not that successful. K. phaffii also displayed the highest bioaccumulation capacity for Cd (25.23 mg/g), Cu (21.63 mg/g), and Pb (20.63 mg/g) at 200 ppm. Scanning electron microscopy (SEM) explored the morphological changes in the mycelia of stressed fungi. Results of this study describe this delicate approach to be species and metal dependent and suggest a potential utilization of this fungal strain for the bioremediation of contaminated soils. |
| 关键词 | heavy metals Komagataella phaffii tolerance index Cd Cr Pb bioaccumulation capacity |
| 学科领域 | Biology |
| DOI | 10.3390/biology9120469 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | ASPERGILLUS-NIGER ; FUSARIUM-WILT ; BIOSORPTION ; COPPER ; EFFLUENT ; REMOVAL ; CADMIUM ; BIOACCUMULATION ; IDENTIFICATION ; RESISTANCE |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS记录号 | WOS:000601683500001 |
| 出版者 | MDPI |
| 文献子类 | Article |
| 出版地 | BASEL |
| EISSN | 2079-7737 |
| 资助机构 | Shanghai Sciences and Technology Commission |
| 作者邮箱 | fizaliaquat@bs.qau.edu.pk ; munis@qau.edu.pk ; uharoonsheikh@gmail.com ; samiaharif24@gmail.com ; saddamsaqib.qau@gmail.com ; shangla123@gmail.com ; alirazakhan.qau@gmail.com ; jianxin.shi@sjtu.edu.cn ; chsq@sjtu.edu.cn ; liuql@sjtu.edu.cn |
| 作品OA属性 | Green Published, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21488 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China 2.Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad 45320, Pakistan 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Zhejiang Key Lab Crop Germplasm, Hangzhou 310058, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China 7.Shanghai Jiao Tong Univ, Sch Design, Dept Landscape Architecture, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liaquat, Fiza,Munis, Muhammad Farooq Hussain,Haroon, Urooj,et al. Evaluation of Metal Tolerance of Fungal Strains Isolated from Contaminated Mining Soil of Nanjing, China[J]. BIOLOGY-BASEL,2020,9(12). |
| APA | Liaquat, Fiza.,Munis, Muhammad Farooq Hussain.,Haroon, Urooj.,Arif, Samiah.,Saqib, Saddam.,...&Liu, Qunlu.(2020).Evaluation of Metal Tolerance of Fungal Strains Isolated from Contaminated Mining Soil of Nanjing, China.BIOLOGY-BASEL,9(12). |
| MLA | Liaquat, Fiza,et al."Evaluation of Metal Tolerance of Fungal Strains Isolated from Contaminated Mining Soil of Nanjing, China".BIOLOGY-BASEL 9.12(2020). |
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| Liaquat-2020-Evaluat（2431KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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