SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening

doi:10.1104/pp.20.01203

	SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening
	Ji, Dongchao 1; Cui, Xiaomin 1; Qin, Guozheng; Chen, Tong ; Tian, Shiping1,2
	2020
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	184 期号:4 页码:2168-2181
摘要	The tomato membrane protein SlFERL regulates fruit ripening via modulating ethylene production. Fruit ripening is a complex and genetically programmed process modulated by transcription factors, hormones, and other regulators. However, the mechanism underlying the regulatory loop involving the membrane-protein targets of RIPENING-INHIBITOR (RIN) remains poorly understood. To unravel the function of tomato (Solanum lycopersicum) FERONIA Like (SlFERL), a putative MADS-box transcription factor target gene, we investigated and addressed the significance of SlFERL in fruit ripening by combining reverse genetics, biochemical, and cytological analyses. Here, we report that RIN and Tomato AGAMOUS-LIKE1 (TAGL1) directly bind to the promoter region of SlFERL and further activate its expression transcriptionally, suggesting a potential role of SlFERL in fruit ripening. Overexpression of SlFERL significantly accelerated the ripening process of tomato fruit, whereas RNA interference knockdown of SlFERL resulted in delayed fruit ripening. Moreover, a surface plasmon resonance assay coupled with tandem mass spectrometry and a protein interaction assay revealed that SlFERL interacts with the key enzyme S-adenosyl-Met synthetase 1 (SlSAMS1) in the ethylene biosynthesis pathway, leading to increased S-adenosyl-Met accumulation and elevated ethylene production. Thus, SlFERL serves as a positive regulator of ethylene production and fruit ripening. This study provides clues to the molecular regulatory networks underlying fruit ripening.
学科领域	Plant Sciences
DOI	10.1104/pp.20.01203
收录类别	SCI
语种	英语
WOS关键词	ADENOSYL-L-METHIONINE ; ETHYLENE BIOSYNTHESIS ; RECEPTOR KINASES ; INHIBITOR RIN ; TOMATO ; FERONIA ; ARABIDOPSIS ; GENE ; REVEAL ; IDENTIFICATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000601303200046
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31925035, 32072637]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21490
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Key Lab Post Harvest Handling Fruits, Minist Agr, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,et al. SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening[J]. PLANT PHYSIOLOGY,2020,184(4):2168-2181.
APA	Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,Chen, Tong,&Tian, Shiping.(2020).SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening.PLANT PHYSIOLOGY,184(4),2168-2181.
MLA	Ji, Dongchao,et al."SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening".PLANT PHYSIOLOGY 184.4(2020):2168-2181.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Ji-2020-SlFERL Inter（1331KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文