Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato

doi:10.1038/s42003-020-01474-3

	Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato
	Wang, Peiwen 1; Wang, Yuying ; Wang, Weihao ; Chen, Tong ; Tian, Shiping1 ; Qin, Guozheng1
	2020
发表期刊	COMMUNICATIONS BIOLOGY
卷号	3 期号:1
摘要	Carotenoids are natural pigments that are indispensable to plants and humans, whereas the regulation of carotenoid biosynthesis by post-translational modification remains elusive. Here, we show that a tomato E3 ubiquitin ligase, Plastid Protein Sensing RING E3 ligase 1 (PPSR1), is responsible for the regulation of carotenoid biosynthesis. PPSR1 exhibits self-ubiquitination activity and loss of PPSR1 function leads to an increase in carotenoids in tomato fruit. PPSR1 affects the abundance of 288 proteins, including phytoene synthase 1 (PSY1), the key rate-limiting enzyme in the carotenoid biosynthetic pathway. PSY1 contains two ubiquitinated lysine residues (Lys380 and Lys406) as revealed by the global analysis and characterization of protein ubiquitination. We provide evidence that PPSR1 interacts with PSY1 precursor protein and mediates its degradation via ubiquitination, thereby affecting the steady-state level of PSY1 protein. Our findings not only uncover a regulatory mechanism for controlling carotenoid biosynthesis, but also provide a strategy for developing carotenoid-enriched horticultural crops. Wang et al. report on the role of a novel E3 ubiquitin ligase, Plastid Protein Sensing RING E3 ligase 1 (PPSR1), during tomato fruit ripening and find that it interacts with phytoene synthase 1 (PSY1) precursor protein and mediates its degradation via ubiquitination. This affects the steady-state level of PSY1 protein, the key rate-limiting enzyme in the carotenoid biosynthetic pathway. This study may provide a strategy for developing carotenoid-enriched horticultural crops.
学科领域	Biology ; Multidisciplinary Sciences
DOI	10.1038/s42003-020-01474-3
收录类别	SCI
语种	英语
WOS关键词	E3 LIGASE ATCHIP ; TRANSCRIPTION FACTOR ; PROTEASOME SYSTEM ; GENE-EXPRESSION ; FRUITFULL HOMOLOGS ; PROTEIN ; DEGRADATION ; ARABIDOPSIS ; ACCUMULATION ; IDENTIFICATION
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
WOS记录号	WOS:000598831000002
出版者	NATURE RESEARCH
文献子类	Article
出版地	BERLIN
EISSN	2399-3642
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31925035, 31930086, 31572174]
作者邮箱	gzqin@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21493
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peiwen,Wang, Yuying,Wang, Weihao,et al. Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato[J]. COMMUNICATIONS BIOLOGY,2020,3(1).
APA	Wang, Peiwen,Wang, Yuying,Wang, Weihao,Chen, Tong,Tian, Shiping,&Qin, Guozheng.(2020).Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato.COMMUNICATIONS BIOLOGY,3(1).
MLA	Wang, Peiwen,et al."Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato".COMMUNICATIONS BIOLOGY 3.1(2020).

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