Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals

doi:10.1016/j.hpj.2020.11.006

	Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals
	Fu, Zhenzhu ; Shang, Hongquan ; Jiang, Hui ; Gao, Jie ; Dong, Xiaoyu ; Wang, Huijuan ; Li, Yanmin ; Wang, Limin ; Zhang, Jing ; Shu, Qingyan 1; Chao, Yacong 2; Xu, Menglan 2; Wang, Rui ; Wang, Liangsheng1 ; Zhang, Hechen
	2020
发表期刊	HORTICULTURAL PLANT JOURNAL
ISSN	2095-9885
卷号	6 期号:6 页码:428-438
摘要	Previous studies have shown that high light intensity can induce anthocyanin synthesis (AS) in petunia plants. To identify which kind of light quality plays a role in inducing such metabolic process, and what transcripts participate in controlling it, we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality conditions. Among the red and white light treatments, a total of 2 205 differentially expressed genes and 15, 22, and 20 differentially expressed circRNAs, miRNAs, and lncRNAs, were identified respectively. The AS-related genes, including the structural genes CHSj, F3'H, F3'5'H, DFR, and ANS , and the regulatory genes AN4, DPL, PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light condition. Furthermore, the light photoreceptor Cryptochrome 3 (CRY3) and a series of light-dependent genes, such as PIF, HY5, and BBXs, were also determined to respond to the light treatments. The anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue light. The results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light quality. Yeast two-hybrid results showed that the key elements in the light signal pathway, HY5 can interact with BBX19, BBX24 and BBX25. And PHZ, the important AS regulator can induce anthocyanin synthesis in response to blue light quality from transient expression analysis in petunia petals. These findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis, but also allow us to explore potential target genes for improving pigment production in petunia flower petals.
关键词	Petunia Anthocyanin Transcription factor Light quality Transcriptome analysis
学科领域	Plant Sciences ; Horticulture
DOI	10.1016/j.hpj.2020.11.006
收录类别	SCI
语种	英语
WOS关键词	B-BOX PROTEIN ; UV-B ; ARABIDOPSIS ; BIOSYNTHESIS ; GENE ; HY5 ; ACCUMULATION ; EXPRESSION ; EVOLUTION ; COP1/SPA
WOS研究方向	Plant Sciences ; Agriculture
WOS记录号	WOS:000597061600008
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	2468-0141
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [U1504320] ; Financial Project of Henan Province [2019ZC23]
作者邮箱	wanglsh@ibcas.ac.cn ; zhc5128@126.com
作品OA属性	gold
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21499
专题	中科院北方资源植物重点实验室
作者单位	1.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China
推荐引用方式 GB/T 7714	Fu, Zhenzhu,Shang, Hongquan,Jiang, Hui,et al. Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals[J]. HORTICULTURAL PLANT JOURNAL,2020,6(6):428-438.
APA	Fu, Zhenzhu.,Shang, Hongquan.,Jiang, Hui.,Gao, Jie.,Dong, Xiaoyu.,...&Zhang, Hechen.(2020).Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals.HORTICULTURAL PLANT JOURNAL,6(6),428-438.
MLA	Fu, Zhenzhu,et al."Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals".HORTICULTURAL PLANT JOURNAL 6.6(2020):428-438.

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