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| Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals | |
Fu, Zhenzhu; Shang, Hongquan; Jiang, Hui; Gao, Jie; Dong, Xiaoyu; Wang, Huijuan; Li, Yanmin; Wang, Limin; Zhang, Jing; Shu, Qingyan1; Chao, Yacong2; Xu, Menglan2; Wang, Rui; Wang, Liangsheng1 ; Zhang, Hechen
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| 2020 | |
| Source Publication | HORTICULTURAL PLANT JOURNAL
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| ISSN | 2095-9885 |
| Volume | 6Issue:6Pages:428-438 |
| Abstract | Previous studies have shown that high light intensity can induce anthocyanin synthesis (AS) in petunia plants. To identify which kind of light quality plays a role in inducing such metabolic process, and what transcripts participate in controlling it, we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality conditions. Among the red and white light treatments, a total of 2 205 differentially expressed genes and 15, 22, and 20 differentially expressed circRNAs, miRNAs, and lncRNAs, were identified respectively. The AS-related genes, including the structural genes CHSj, F3'H, F3'5'H, DFR, and ANS , and the regulatory genes AN4, DPL, PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light condition. Furthermore, the light photoreceptor Cryptochrome 3 (CRY3) and a series of light-dependent genes, such as PIF, HY5, and BBXs, were also determined to respond to the light treatments. The anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue light. The results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light quality. Yeast two-hybrid results showed that the key elements in the light signal pathway, HY5 can interact with BBX19, BBX24 and BBX25. And PHZ, the important AS regulator can induce anthocyanin synthesis in response to blue light quality from transient expression analysis in petunia petals. These findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis, but also allow us to explore potential target genes for improving pigment production in petunia flower petals. |
| Keyword | Petunia Anthocyanin Transcription factor Light quality Transcriptome analysis |
| Subject Area | Plant Sciences ; Horticulture |
| DOI | 10.1016/j.hpj.2020.11.006 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | B-BOX PROTEIN ; UV-B ; ARABIDOPSIS ; BIOSYNTHESIS ; GENE ; HY5 ; ACCUMULATION ; EXPRESSION ; EVOLUTION ; COP1/SPA |
| WOS Research Area | Plant Sciences ; Agriculture |
| WOS ID | WOS:000597061600008 |
| Publisher | ELSEVIER |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 2468-0141 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [U1504320] ; Financial Project of Henan Province [2019ZC23] |
| Corresponding Author Email | wanglsh@ibcas.ac.cn ; zhc5128@126.com |
| OA | gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21499 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China |
| Recommended Citation GB/T 7714 | Fu, Zhenzhu,Shang, Hongquan,Jiang, Hui,et al. Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals[J]. HORTICULTURAL PLANT JOURNAL,2020,6(6):428-438. |
| APA | Fu, Zhenzhu.,Shang, Hongquan.,Jiang, Hui.,Gao, Jie.,Dong, Xiaoyu.,...&Zhang, Hechen.(2020).Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals.HORTICULTURAL PLANT JOURNAL,6(6),428-438. |
| MLA | Fu, Zhenzhu,et al."Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals".HORTICULTURAL PLANT JOURNAL 6.6(2020):428-438. |
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| 1-s2.0-S246801412030（3671KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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