Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress

doi:10.1371/journal.pone.0243112

	Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress
	Li, Qian ; Ma, Changkun 1; Tai, Huanhuan 2; Qiu, Huan ; Yang, An 3
	2020
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	15 期号:12
摘要	Saline-alkaline stress is an abiotic stress that suppresses rice plant growth and reduces yield. However, few studies have investigated the mechanism by which rice plants respond to saline-alkaline stress at a global transcriptional level. Dongdao-4 and Jigeng-88, which differ in their tolerance to saline-alkaline stress, were used to explore gene expression differences under saline-alkaline stress by RNA-seq technology. In seedlings of Dongdao-4 and Jigeng-88, 3523 and 4066 genes with differential levels of expression were detected, respectively. A total of 799 genes were upregulated in the shoots of both Dongdao-4 and Jigeng-88, while 411 genes were upregulated in the roots of both genotypes. Among the downregulated genes in Dongdao-4 and Jigeng-88, a total of 453 and 372 genes were found in shoots and roots, respectively. Gene ontology (GO) analysis showed that upregulated genes were enriched in several GO terms such as response to stress, response to jasmonic acid, organic acid metabolic process, nicotianamine biosynthetic process, and iron homeostasis. The downregulated genes were enriched in several GO terms, such as photosynthesis and response to reactive oxygen species. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that Dongdao-4 seedlings were specifically enriched in the biosynthesis of secondary metabolites such as diterpenoids and phenylpropanoids. The upregulated genes that were involved in secondary metabolite biosynthesis, amino acid biosynthesis, betalain biosynthesis, organic acid metabolic process, and iron homeostasis pathways may be central to saline-alkaline tolerance in both rice genotypes. In contrast, the genes involved in the diterpenoid and phenylpropanoid biosynthesis pathways may contribute to the greater tolerance to saline-alkaline stress in Dongdao-4 seedlings than in Jigeng-88. These results suggest that Dongdao-4 was equipped with a more efficient mechanism involved in multiple biological processes to adapt to saline-alkaline stress.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0243112
收录类别	SCI
语种	英语
WOS关键词	GENOME-WIDE ANALYSIS ; SALT TOLERANCE ; SEROTONIN BIOSYNTHESIS ; GREATER TOLERANCE ; RESPONSES ; DROUGHT ; GENE ; IRON ; FAMILY ; OVEREXPRESSION
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000596503200040
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
作者邮箱	anyang@ibcas.ac.cn ; liqian18@nwafu.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21502
专题	植被与环境变化国家重点实验室
作者单位	1.Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China 2.Xian Univ Technol, State Key Lab Ecohydraul Engn Arid Area, Xian, Peoples R China 3.Northwest A&F Univ, Coll Agron, Yangling, Shaanxi, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Ma, Changkun,Tai, Huanhuan,et al. Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress[J]. PLOS ONE,2020,15(12).
APA	Li, Qian,Ma, Changkun,Tai, Huanhuan,Qiu, Huan,&Yang, An.(2020).Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress.PLOS ONE,15(12).
MLA	Li, Qian,et al."Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress".PLOS ONE 15.12(2020).

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