The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development

doi:10.1111/tpj.15024

	The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development
	Liu, Jianyang 1; Wang, Yanning 1; Cheng, Youfa1,2
	2020
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	104 期号:6 页码:1617-1634
摘要	The highly conserved endosomal sorting complex required for transport (ESCRT) pathway plays critical roles in endosomal sorting of ubiquitinated plasma membrane proteins for degradation. However, the functions of many components of the ESCRT machinery in plants remain unsolved. Here we show that the ESCRT-I subunits VPS28A and VPS28B are functionally redundant and required for embryonic development in Arabidopsis. We conducted a screen for genetic enhancers of pid, which is defective in auxin signaling and transport. We isolated a no--cotyledon in pid 104 (ncp104) mutant, which failed to develop cotyledons in a pid background. We discovered that ncp104 was a unique recessive gain-of-function allele of vps28a. VPS28A and VPS28B were expressed during embryogenesis and the proteins were localized to the trans-Golgi network/early endosome and post-Golgi/endosomal compartments, consistent with their functions in endosomal sorting and embryogenesis. The single vps28a and vps28b loss-of-function mutants did not display obvious developmental defects, but their double mutants showed abnormal cell division patterns and were arrested at the globular embryo stage. The vps28a vps28b double mutants showed altered auxin responses, disrupted PIN1-GFP expression patterns, and abnormal PIN1-GFP accumulation in small aberrant vacuoles. The ncp104 mutation may cause the VPS28A protein to become unstable and/or toxic. Taken together, our findings demonstrate that the ESCRT-I components VPS28A and VPS28B redundantly play essential roles in vacuole formation, endosomal sorting of plasma membrane proteins, and auxin-mediated plant development.
关键词	VPS28 endosomal sorting complex required for transport auxin endosomal sorting embryogenesis vacuole
学科领域	Plant Sciences
DOI	10.1111/tpj.15024
收录类别	SCI
语种	英语
WOS关键词	PROTEIN-PROTEIN INTERACTIONS ; YUCCA FLAVIN MONOOXYGENASES ; MULTIVESICULAR BODIES ; ARABIDOPSIS ; TRANSPORT ; COMPLEX ; BIOSYNTHESIS ; REVEALS ; LOCALIZATION ; TRAFFICKING
WOS研究方向	Plant Sciences
WOS记录号	WOS:000587141100001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [31970309, 31171389, 91217310, 91017008, 31270330] ; National Key Research and Development Program of China [2016YFD0100402] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; Chinese Academy of Sciences STS Program [KFJ-STS-ZDTP-076]
作者邮箱	yfcheng@ibcas.ac.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21528
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jianyang,Wang, Yanning,Cheng, Youfa. The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development[J]. PLANT JOURNAL,2020,104(6):1617-1634.
APA	Liu, Jianyang,Wang, Yanning,&Cheng, Youfa.(2020).The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development.PLANT JOURNAL,104(6),1617-1634.
MLA	Liu, Jianyang,et al."The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development".PLANT JOURNAL 104.6(2020):1617-1634.

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