Structural basis for energy transfer in a huge diatom PSI-FCPI supercomplex

doi:10.1038/s41467-020-18867-x

IB-CAS > 中科院光生物学重点实验室

	Structural basis for energy transfer in a huge diatom PSI-FCPI supercomplex
	Xu, Caizhe 1; Pi, Xiong ; Huang, Yawen ; Han, Guangye ; Chen, Xiaobo 4; Qin, Xiaochun 5; Huang, Guoqiang ; Zhao, Songhao 1; Yang, Yanyan ; Kuang, Tingyun ; Wang, Wenda ; Sui, Sen-Fang ; Shen, Jian-Ren
	2020
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	11 期号:1
摘要	Diatom is an important group of marine algae and contributes to around 20% of the global photosynthetic carbon fixation. Photosystem I (PSI) of diatoms is associated with a large number of fucoxanthin-chlorophyll a/c proteins (FCPIs). We report the structure of PSI-FCPI from a diatom Chaetoceros gracilis at 2.38 angstrom resolution by single-particle cryo-electron microscopy. PSI-FCPI is a monomeric supercomplex consisting of 12 core and 24 antenna subunits (FCPIs), and 326 chlorophylls a, 34 chlorophylls c, 102 fucoxanthins, 35 diadinoxanthins, 18 beta -carotenes and some electron transfer cofactors. Two subunits designated PsaR and PsaS were found in the core, whereas several subunits were lost. The large number of pigments constitute a unique and huge network ensuring efficient energy harvesting, transfer and dissipation. These results provide a firm structural basis for unraveling the mechanisms of light-energy harvesting, transfer and quenching in the diatom PSI-FCPI, and also important clues to evolutionary changes of PSI-LHCI. Diatoms are marine algae with an important role in global photosynthetic carbon fixation. Here, the authors present the 2.38 angstrom cryo-EM structure of photosystem I (PSI) in complex with its 24 fucoxanthin chlorophyll a/c-binding (FCPI) antenna proteins from the diatom Chaetoceros gracilis, which provides mechanistic insights into light-energy harvesting, transfer and quenching of the PSI-FCPI supercomplex.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41467-020-18867-x
收录类别	SCI
语种	英语
WOS关键词	FUCOXANTHIN-CHLOROPHYLL-PROTEINS ; PHOTOSYSTEM-I SUPERCOMPLEX ; LIGHT-HARVESTING COMPLEX ; TRANSFER PATHWAYS ; BINDING PROTEINS ; EVOLUTION ; GENOME ; ALGA
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000581918200012
出版者	NATURE RESEARCH
文献子类	Article
出版地	BERLIN
资助机构	National Key R&D Program of China [2017YFA0503700, 2017YFA0504600, 2019YFA0906300, 2016YFA0501101] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31970260, 31600191, 31861143048, 31670745] ; Strategic Priority Research Program of CAS [XDB17000000] ; CAS Key Research Program for Frontier Science [QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS [2020081]
作者邮箱	wdwang@ibcas.ac.cn ; suisf@mail.tsinghua.edu.cn ; jrshen@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21542
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, State Key Lab Membrane Biol, Beijing 100084, Peoples R China 4.Tsinghua Univ, Sch Life Sci, Frontier Res Ctr Biol Struct, Beijing 100084, Peoples R China 5.Hebei Univ Sci & Technol, Sch Biol Sci & Engn, Shijiazhuang 050018, Hebei, Peoples R China 6.Jinan Univ, Sch Biol Sci & Technol, Jinan 250022, Peoples R China 7.Southen Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China 8.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan 9.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Xu, Caizhe,Pi, Xiong,Huang, Yawen,et al. Structural basis for energy transfer in a huge diatom PSI-FCPI supercomplex[J]. NATURE COMMUNICATIONS,2020,11(1).
APA	Xu, Caizhe.,Pi, Xiong.,Huang, Yawen.,Han, Guangye.,Chen, Xiaobo.,...&Shen, Jian-Ren.(2020).Structural basis for energy transfer in a huge diatom PSI-FCPI supercomplex.NATURE COMMUNICATIONS,11(1).
MLA	Xu, Caizhe,et al."Structural basis for energy transfer in a huge diatom PSI-FCPI supercomplex".NATURE COMMUNICATIONS 11.1(2020).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xu-2020-Structural b（4606KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文