Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection

doi:10.1371/journal.ppat.1008919

	Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection
	Ding, Yijuan 1; Mei, Jiaqin 1; Chai, Yaru 2; Yang, Wenjing 1; Mao, Yi 1; Yan, Baoqin 1; Yu, Yang 3; Disi, Joseph Onwusemu 4; Rana, Kusum 1; Li, Jiana 1; Qian, Wei 1
	2020
发表期刊	PLOS PATHOGENS
ISSN	1553-7366
卷号	16 期号:10
摘要	Necrotrophic plant pathogen induces host reactive oxygen species (ROS) production, which leads to necrosis in the host, allowing the pathogen to absorb nutrients from the dead tissues.Sclerotinia sclerotiorumis a typical necrotrophic pathogen that causes Sclerotinia stem rot in more than 400 species, resulting in serious economic losses. Here, we found that threeS.sclerotiorumgenes involved in copper ion import/transport,SsCTR1,SsCCSandSsATX1, were significantly up-regulated during infection ofBrassica oleracea. Function analysis revealed that these genes involved in fungal ROS detoxification and virulence. On the host side, four genes putatively involved in copper ion homeostasis,BolCCS,BolCCH,BolMT2AandBolDRT112, were significantly down-regulated in susceptibleB.oleracea, but stably expressed in resistantB.oleraceaduring infection. Their homologs were found to promote resistance toS.sclerotiorumand increase antioxidant activity inArabidopsis thaliana. Furthermore, copper concentration analysis indicated that copper flow from healthy area into the necrotic area during infection. A model was proposed thatS.sclerotiorumutilizes host copper to detoxify ROS in its cells, whereas the resistant hosts may restrict the supply of essential copper nutrients toS.sclerotiorumby maintaining copper ion homeostasis during infection. Author summary Sclerotinia sclerotiorumis a devastating necrotrophic fungal pathogen and has a substantial economic impact on crop production worldwide.S.sclerotiorumsuccessfully infect the host plants by promote ROS production in host and induce plant necrosis. This raises an interesting question of how this pathogen survive from high level of host-derived ROS. Copper is an essential nutrient for microbial pathogens and serves as an important cofactor for enzymes of scavenging ROS. In our previous study, we simultaneously analyzed the transcriptome changes ofBrassica oleraceaandS.sclerotiorumduring inoculation. The results in present study show thatS.sclerotioruminduces the genes involved in 'copper ion import' and 'copper ion transport' during infection to promote the ROS detoxification and fungal virulence. While, the resistance host maintains the genes involved in 'copper ion homeostasis', which promote the resistance toS.sclerotiorumand antioxidant activity. Furthermore, copper concentration is higher in the necrotic area than the margin and healthy area, which may indicate that copper flow from healthy area into the necrotic area during infection. In summary, our results highlight a more subtle and complex role of copper during the interaction ofS.sclerotiorumand its host plants.
学科领域	Microbiology ; Parasitology ; Virology
DOI	10.1371/journal.ppat.1008919
收录类别	SCI
语种	英语
WOS关键词	DIFFERENTIAL EXPRESSION ANALYSIS ; CYTOCHROME-C-OXIDASE ; BRASSICA-NAPUS L. ; SUPEROXIDE-DISMUTASE ; NUTRITIONAL IMMUNITY ; ATX1 GENE ; TOLERANCE ; HOMEOSTASIS ; VIRULENCE ; CHAPERONE
WOS研究方向	Microbiology ; Parasitology ; Virology
WOS记录号	WOS:000577091200004
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
EISSN	1553-7374
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671726, 31801395, 31971978] ; Natural Science Foundation of ChongqingNatural Science Foundation of Chongqing [cstc2017shmsxdny80050, cstc2019jcyj-zdxmX0012, cstc2019jcyj-msxmX0486] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [XDJK2018AA004, XDJK2018B022]
作者邮箱	qianwei666@hotmail.com
作品OA属性	Green Published, gold
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21558
专题	中科院植物分子生理学重点实验室
作者单位	1.Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China 2.Southwest Univ, Acad Agr Sci, Chongqing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 4.Southwest Univ, Coll Plant Protect, Chongqing, Peoples R China 5.Univ Georgia, Dept Entomol, Athens, GA 30602 USA
推荐引用方式 GB/T 7714	Ding, Yijuan,Mei, Jiaqin,Chai, Yaru,et al. Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection[J]. PLOS PATHOGENS,2020,16(10).
APA	Ding, Yijuan.,Mei, Jiaqin.,Chai, Yaru.,Yang, Wenjing.,Mao, Yi.,...&Qian, Wei.(2020).Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection.PLOS PATHOGENS,16(10).
MLA	Ding, Yijuan,et al."Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection".PLOS PATHOGENS 16.10(2020).

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