Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner

doi:10.1093/jxb/eraa352

	Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner
	Li, Jinjin 1; Yuan, Jinhong 1; Wang, Hui ; Zhang, Hui 2; Zhang, Haiyan
	2020
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	71 期号:19 页码:6174-6186
摘要	The essential nutrient copper is toxic in excess. Therefore, plants must tightly control copper uptake and distribution. Arabidopsis thaliana high-affinity copper transporters (COPTs) mediate copper uptake, partitioning, and redistribution. Here we show that COPT1 localizes to the plasma membrane and endoplasmic reticulum in stably transgenic plants expressing a COPT1-green fluorescent protein (GFP) fusion protein, and the fusion protein is rapidly degraded upon plant exposure to excess copper. MG132 treatment largely abolished copper-induced degradation of COPT1, implying a link between the proteasome and COPT1 activity in modulating copper uptake. Co-immunoprecipitation analyses revealed that COPT1 cannot be ubiquitinated in the presence of excess copper and MG132. Through site-directed mutagenesis, we identified Lys159 in the C-terminal cytoplasmic tail of COPT1 as critical for copper acquisition, but not for copper-mediated down-regulation of COPT1, in plants. Furthermore, pharmacological analysis showed that treatment with a vesicle trafficking inhibitor or a V-ATPase inhibitor does not alter the subcellular dynamics of COPT1-GFP, consistent with the absence of a connection between the endosomal recycling/vacuolar system and COPT1 degradation. Together, our data suggest that proteasomal degradation rather than vacuolar proteolysis is important for the regulation of copper transport to maintain copper homeostasis in plants.
关键词	Arabidopsis copper transporter degradation proteasome ubiquitination vesicle trafficking
学科领域	Plant Sciences
DOI	10.1093/jxb/eraa352
收录类别	SCI
语种	英语
WOS关键词	PLASMA-MEMBRANE ; AUXIN TRANSPORT ; PROTEIN ; ENDOCYTOSIS ; TRAFFICKING ; HOMEOSTASIS ; GROWTH ; IDENTIFICATION ; ABSENCE ; CELLS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000577075400038
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470292] ; Natural Science Foundation of TianjinNatural Science Foundation of Tianjin [18JCZDJC97900]
作者邮箱	skyzhy@tjnu.edu.cn
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21559
专题	中科院植物分子生理学重点实验室
作者单位	1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jinjin,Yuan, Jinhong,Wang, Hui,et al. Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):6174-6186.
APA	Li, Jinjin,Yuan, Jinhong,Wang, Hui,Zhang, Hui,&Zhang, Haiyan.(2020).Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner.JOURNAL OF EXPERIMENTAL BOTANY,71(19),6174-6186.
MLA	Li, Jinjin,et al."Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):6174-6186.

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