Knowledge Management System Of Institute Of Botany,CAS
A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening | |
Li, Xuyan; Hou, Yanming; Xie, Xin; Li, Hongxue; Li, Xiaodong1; Zhu, Yan1; Zhai, Lulu; Zhang, Chunyu; Bian, Shaomin | |
2020 | |
发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY |
ISSN | 0022-0957 |
卷号 | 71期号:19页码:5976-5989 |
摘要 | Color change is an important event during fruit maturation in blueberry, usually depending on chlorophyll degradation and anthocyanin accumulation. MicroRNA156 (miR156)-SPL modules are an important group of regulatory hubs involved in the regulation of anthocyanin biosynthesis. However, little is known regarding their roles in blueberry or in chlorophyll metabolism during color change. In this study, a MIR156 gene (VcMIR156a) was experimentally identified in blueberry (Vaccinium corymbosum). Overexpression of VcMIR156a in tomato (Solanum lycopersicum) enhanced anthocyanin biosynthesis and chlorophyll degradation in the stem by altering pigment-associated gene expression. Further investigation indicated that the VcSPL12 transcript could be targeted by miR156, and showed the reverse accumulation patterns during blueberry fruit development and maturation. Noticeably, VcSPL12 was highly expressed at green fruit stages, while VcMIR156a transcripts mainly accumulated at the white fruit stage when expression of VcSPL12 was dramatically decreased, implying that VcMIR156a-VcSPL12 is a key regulatory hub during fruit coloration. Moreover, VcSPL12 decreased the expression of several anthocyanin biosynthetic and regulatory genes, and a yeast two-hybrid assay indicated that VcSPL12 interacted with VcMYBPA1. Intriguingly, expression of VcSPL12 significantly enhanced chlorophyll accumulation and altered the expression of several chlorophyll-associated genes. Additionally, the chloroplast ultrastructure was altered by the expression of VcMIR156a and VcSPL12. These findings provide a novel insight into the functional roles of miR156-SPLs in plants, especially in blueberry fruit coloration. |
关键词 | Anthocyanin biosynthesis blueberry chlorophyll accumulation color change fruit development and maturation miR156-SPL module |
学科领域 | Plant Sciences |
DOI | 10.1093/jxb/eraa327 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ABSCISIC-ACID ; CHLOROPLAST DEVELOPMENT ; NEGATIVE REGULATION ; GENE-EXPRESSION ; TOMATO FRUIT ; ARABIDOPSIS ; BIOSYNTHESIS ; ACTIVATION ; DEGRADATION ; SENESCENCE |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000577075400023 |
出版者 | OXFORD UNIV PRESS |
文献子类 | Article |
出版地 | OXFORD |
EISSN | 1460-2431 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872075] |
作者邮箱 | shmbian@jlu.edu.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21560 |
专题 | 植物园 |
作者单位 | 1.Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Xuyan,Hou, Yanming,Xie, Xin,et al. A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):5976-5989. |
APA | Li, Xuyan.,Hou, Yanming.,Xie, Xin.,Li, Hongxue.,Li, Xiaodong.,...&Bian, Shaomin.(2020).A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening.JOURNAL OF EXPERIMENTAL BOTANY,71(19),5976-5989. |
MLA | Li, Xuyan,et al."A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):5976-5989. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Li-2020-A blueberry (3256KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论