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A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening
Li, Xuyan; Hou, Yanming; Xie, Xin; Li, Hongxue; Li, Xiaodong1; Zhu, Yan1; Zhai, Lulu; Zhang, Chunyu; Bian, Shaomin
2020
发表期刊JOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
卷号71期号:19页码:5976-5989
摘要Color change is an important event during fruit maturation in blueberry, usually depending on chlorophyll degradation and anthocyanin accumulation. MicroRNA156 (miR156)-SPL modules are an important group of regulatory hubs involved in the regulation of anthocyanin biosynthesis. However, little is known regarding their roles in blueberry or in chlorophyll metabolism during color change. In this study, a MIR156 gene (VcMIR156a) was experimentally identified in blueberry (Vaccinium corymbosum). Overexpression of VcMIR156a in tomato (Solanum lycopersicum) enhanced anthocyanin biosynthesis and chlorophyll degradation in the stem by altering pigment-associated gene expression. Further investigation indicated that the VcSPL12 transcript could be targeted by miR156, and showed the reverse accumulation patterns during blueberry fruit development and maturation. Noticeably, VcSPL12 was highly expressed at green fruit stages, while VcMIR156a transcripts mainly accumulated at the white fruit stage when expression of VcSPL12 was dramatically decreased, implying that VcMIR156a-VcSPL12 is a key regulatory hub during fruit coloration. Moreover, VcSPL12 decreased the expression of several anthocyanin biosynthetic and regulatory genes, and a yeast two-hybrid assay indicated that VcSPL12 interacted with VcMYBPA1. Intriguingly, expression of VcSPL12 significantly enhanced chlorophyll accumulation and altered the expression of several chlorophyll-associated genes. Additionally, the chloroplast ultrastructure was altered by the expression of VcMIR156a and VcSPL12. These findings provide a novel insight into the functional roles of miR156-SPLs in plants, especially in blueberry fruit coloration.
关键词Anthocyanin biosynthesis blueberry chlorophyll accumulation color change fruit development and maturation miR156-SPL module
学科领域Plant Sciences
DOI10.1093/jxb/eraa327
收录类别SCI
语种英语
WOS关键词ABSCISIC-ACID ; CHLOROPLAST DEVELOPMENT ; NEGATIVE REGULATION ; GENE-EXPRESSION ; TOMATO FRUIT ; ARABIDOPSIS ; BIOSYNTHESIS ; ACTIVATION ; DEGRADATION ; SENESCENCE
WOS研究方向Plant Sciences
WOS记录号WOS:000577075400023
出版者OXFORD UNIV PRESS
文献子类Article
出版地OXFORD
EISSN1460-2431
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872075]
作者邮箱shmbian@jlu.edu.cn
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21560
专题植物园
作者单位1.Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Li, Xuyan,Hou, Yanming,Xie, Xin,et al. A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):5976-5989.
APA Li, Xuyan.,Hou, Yanming.,Xie, Xin.,Li, Hongxue.,Li, Xiaodong.,...&Bian, Shaomin.(2020).A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening.JOURNAL OF EXPERIMENTAL BOTANY,71(19),5976-5989.
MLA Li, Xuyan,et al."A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):5976-5989.
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