Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea

doi:10.1111/njb.02794

	Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea
	Guan, Zheng-Jun ; Shen, Bao-Cheng ; Wei, Wei ; Tang, Zhi-Xi ; Stewart, C. Neal, Jr.
	2020
发表期刊	NORDIC JOURNAL OF BOTANY
ISSN	0107-055X
卷号	38 期号:9
摘要	Transgene flow from engineeredBrassica napusto wild weed relatives could potentially have an environmental effect. To evaluate the introgression of transgenicB. napusinto wildBrassica juncea, the hybrid F(1)and backcross progenies derived fromB. juncea(genome constitution AABB) and transgenicB. napus(AACC) crosses were investigated. C-genome-specific simple sequence repeat (SSR) markers corresponding to linkage groups N11-N19 in B. napuswere screened and used to estimate the marker frequency in hybrid F(1)and backcross progenies. C-genome-specific markers could be stably detected in hybrid F-1 and backcross BC1 plants, but were only rarely found in the BC2-BC5 generations. For example, a specific SSR marker for linkage group N12 segregated in BC2 generation but were completely lost in BC3-BC5, while a specific SSR marker of linkage group N15 segregated in BC1, BC2 and BC3 generations and was absent in more advanced backcrossed generations (BC4 and BC5). The results indicate that a certain gene regions inBrassica napusplants are transmitted at a relatively lower frequency to wild relatives, and more rapidly disappeared in subsequent backcross generations. We propose that a foreign gene or transgene that is integrated in the C-chromosome ofBrassica napuscould reduce the risk of introgression in nature.
关键词	biosafety Brassica juncea Brassica napus transgene introgression
学科领域	Plant Sciences
DOI	10.1111/njb.02794
收录类别	SCI
语种	英语
WOS关键词	OILSEED RAPE ; MUSTARD POPULATIONS ; GENE FLOW ; L. CZERN ; HYBRIDS ; RECOMBINATION ; EXPRESSION ; DIVERSITY ; PERSIST ; FITNESS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000576620000001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1756-1051
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200422, 31370357] ; Key Disciplines Construction Foundation of the 1331 Project of Shanxi Province [098-091704] ; Natural Science Foundation of Shanxi Province in ChinaNatural Science Foundation of Shanxi Province [201701D121089] ; Yuncheng University [YQ-2014022, XK-2019001]
作者邮箱	weiwei@ibcas.ac.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21565
专题	植被与环境变化国家重点实验室
作者单位	1.Yuncheng Univ, Dept Life Sci, Yuncheng, Shanxi, Peoples R China 2.[Shen, Bao-Cheng 3.Wei, Wei 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, CN-100093 Beijing, Peoples R China 5.[Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN USA
推荐引用方式 GB/T 7714	Guan, Zheng-Jun,Shen, Bao-Cheng,Wei, Wei,et al. Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea[J]. NORDIC JOURNAL OF BOTANY,2020,38(9).
APA	Guan, Zheng-Jun,Shen, Bao-Cheng,Wei, Wei,Tang, Zhi-Xi,&Stewart, C. Neal, Jr..(2020).Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea.NORDIC JOURNAL OF BOTANY,38(9).
MLA	Guan, Zheng-Jun,et al."Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea".NORDIC JOURNAL OF BOTANY 38.9(2020).

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