IB-CAS  > 植被与环境变化国家重点实验室
Physiological and symbiotic variation of a long-term evolved Rhizobium strain under alkaline condition
Ji, Zhao Jun1,2,3,4; Wu, Zhong Yu1,2; Chen, Wen Feng1,2; Wang, En Tao5; Yan, Hui6; Cui, Qing Guo7; Zhang, Ji Xing3,4; Wang, Lei1,2; Ma, Sheng Jun8
2020
Source PublicationSYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN0723-2020
Volume43Issue:5
AbstractPhysiological variation and adaptation of the long-term evolved rhizobia to alkaline environments where no host plant existence and the stability of their symbiotic properties when they are reinoculated to legume host remain unclear. A highly effective N-2-fixing Rhizobium yanglingense strain CCBAU 01603 was used as the ancestral strain and was cultured continuously with/without addition of extra alkaline reagent (KOH) in laboratory conditions for approximately 500 generations. Total 60 evolved clones obtained were checked for their adaptation to higher alkaline pH level and inoculated to their host plant Caragana microphylla to evaluate their symbiotic efficiencies. Most of the evolved clones showed increased adaptation to higher alkaline pH but all of them decreased symbiotic efficiencies, resulting in the formation of irregular root nodules with lower nitrogenase activity, production of abnormal bacteroids, and accumulation more starch grains in uninfected nodule cells. Further demonstration of lower symbiotic efficiencies came from the down-regulated expression of genes related to nitrogen fixation in the bacteroids by transcriptome comparison. In addition, genes related to transporters and other diverse functions were up- or down-regulated in the evolved clones in free-living conditions (like yjiS gene) or in symbiotic situations, demonstrating the significant variations in cellular physiology and symbiosis. Our study revealed that the enhancement of alkaline adaptation but loss of symbiotic efficiencies of the evolved clones had happened during the long-term evolution in alkaline environments where no selective pressures from host plant, offering new insight into the molecular mechanism and direction of rhizobial evolution in nature. (C) 2020 Elsevier GmbH. All rights reserved.
KeywordExperimental evolution Rhizobium Symbiosis Nodulation Alkaline environments
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
DOI10.1016/j.syapm.2020.126125
Indexed BySCI
Language英语
WOS KeywordCARAGANA SPP. ; EVOLUTION ; ADAPTATION ; NODULATION ; DIVERSITY ; GENES ; SOIL
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000574879000006
PublisherELSEVIER GMBH
SubtypeArticle
Publication PlaceMUNICH
EISSN1618-0984
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31860001, 31770039] ; Natural Science Foundation of Inner Mongolia Autonomous Region of China [2017MS(LH)0306] ; Research and Demonstration of Local Alfalfa Rhizobium Products in Tibet [XZ201801NB20] ; Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region [NJYT-19-Al 0] ; Chinese Universities Scientific Fund [2019TC161] ; National Institute of Polytechnology, Mexico ; [SIP20195271]
Corresponding Author Emailchenwf@cau.edu.cn
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21568
Collection植被与环境变化国家重点实验室
Affiliation1.State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China
3.China Agr Univ, Rhizobium Res Ctr, Beijing 100193, Peoples R China
4.Inner Mongolia Univ Nationalities, Coll Life Sci, Tongliao 028042, Inner Mongolia, Peoples R China
5.Inner Mongolia Univ Nationalities, Horqin Plant Stress Biol Res Inst, Tongliao 028042, Inner Mongolia, Peoples R China
6.Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City 11340, DF, Mexico
7.Agr Univ Hebei, Coll Anim Sci & Technol, Baoding 071000, Hebei, Peoples R China
8.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
9.Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Xinjiang Uygur, Peoples R China
Recommended Citation
GB/T 7714
Ji, Zhao Jun,Wu, Zhong Yu,Chen, Wen Feng,et al. Physiological and symbiotic variation of a long-term evolved Rhizobium strain under alkaline condition[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY,2020,43(5).
APA Ji, Zhao Jun.,Wu, Zhong Yu.,Chen, Wen Feng.,Wang, En Tao.,Yan, Hui.,...&Ma, Sheng Jun.(2020).Physiological and symbiotic variation of a long-term evolved Rhizobium strain under alkaline condition.SYSTEMATIC AND APPLIED MICROBIOLOGY,43(5).
MLA Ji, Zhao Jun,et al."Physiological and symbiotic variation of a long-term evolved Rhizobium strain under alkaline condition".SYSTEMATIC AND APPLIED MICROBIOLOGY 43.5(2020).
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