A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing

doi:10.1186/s12284-020-00425-0

	A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing
	Ge, Qiang 1,2; Tang, Yongyan ; Luo, Wei ; Zhang, Jingyu ; Chong, Kang 3; Xu, Yunyuan 3
	2020
发表期刊	RICE
ISSN	1939-8425
卷号	13 期号:1
摘要	Background Grain size is one of the key agronomic traits that impact grain yield. Several regulatory pathways had been reported to participate in grain size determination via cell expansion or proliferation in rice. However, little is known about cyclophilin and spliceosome participation in grain shape regulation. Results Here, we identified OsCYP20-2, a cyclophilin that influences spliceosome assembly to determine grain length.oscyp20-2 t1, a knock out mutant ofOsCYP20-2caused by T-DNA insertion, produced shorter grains with deficient cell elongation. Through yeast two-hybrid screening and pull-down assays, OsSYF2, a pre-mRNA splicing factor, was identified as an interacting protein of OsCYP20-2. The phenotypes of transgenic lines indicated thatOsSYF2positively regulates grain length via its influence on cell expansion. Transcriptomic analysis showed thatOsSYF2controls the expression and pre-mRNA alternative splicing of genes involved in sugar metabolism. In addition, these two genes have similar effects on panicle architecture. Conclusions Taken together, OsSYF2, an interacting protein of OsCYP20-2, controls grain length and panicle architecture by regulating the alternative splicing of pre-mRNA involved in cell elongation and sugar metabolism.
关键词	OsCYP20-2 Grain length OsSYF2 Spliceosome Alternative splicing
学科领域	Agronomy
DOI	10.1186/s12284-020-00425-0
收录类别	SCI
语种	英语
WOS关键词	NATURAL VARIATION ; GENE-EXPRESSION ; RICE ; PROTEIN ; ENCODES ; ARABIDOPSIS ; SIZE ; IDENTIFICATION ; WEIGHT ; YIELD
WOS研究方向	Agriculture
WOS记录号	WOS:000571813300001
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1939-8433
资助机构	National Key Research and Development Program [2016YFD0100901]
作者邮箱	xuyy@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21579
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Agr Univ, Coll Agron, Zhengzhou 450046, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Ge, Qiang,Tang, Yongyan,Luo, Wei,et al. A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing[J]. RICE,2020,13(1).
APA	Ge, Qiang,Tang, Yongyan,Luo, Wei,Zhang, Jingyu,Chong, Kang,&Xu, Yunyuan.(2020).A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing.RICE,13(1).
MLA	Ge, Qiang,et al."A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing".RICE 13.1(2020).

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